We narrowed to 2,187 results for: AP1;

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pcDNA6.2EmGFP-DAXX

Plasmid

#169726

Purpose

Mammalian expression construct of GFP tagged full length DAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX (DAXX Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX:PGRMC1

Plasmid

#136062

Purpose

Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.

Depositor

Francisco Domínguez Hernández

Article

Salsano et al Fertil Steril. 2020 May;113(5):1050-1066

Insert

Progesterone receptor membrane component 1 (PGRMC1 Human)

Tags

GST-PGRMC1

Expression

Bacterial

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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TRIM28 gRNA (BRDN0001162487)

Plasmid

#76139

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM28

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM28 (TRIM28 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIM28 gRNA (BRDN0001146162)

Plasmid

#76142

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM28

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM28 (TRIM28 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP2-1st WW mutant

Plasmid

#19046

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein isoform 3 (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine,and Proline 202…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP2-2nd WW mutant

Plasmid

#19047

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 258 changed to Alanine, and Proline 2…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3-EMTB-TurboID-V5

Plasmid

#190736

Purpose

Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

ensconsin (MAP7 Human)

Tags

Myc, TurboID, and V5

Expression

Mammalian

Mutation

Microtubule binding-domain of ensconsin (amino ac…

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDsRed Monomer C1-YAP2-S127A mutant

Plasmid

#19058

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

Red-FPP

Expression

Mammalian

Mutation

Serine 127 changed to Alanine

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
FKBP-beta2(616-951)-GFP

Plasmid

#100726

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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HA-YAP-wt

Plasmid

#229412

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

YAP-wt (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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TRIM28 gRNA (BRDN0001145369)

Plasmid

#76141

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM28

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM28 (TRIM28 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-mScarlet-Dync1h1motor

Plasmid

#191330

Purpose

Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-cDAXX

Plasmid

#169729

Purpose

Bacterial expression of N-terminally 6His tagged cDAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX (DAXX Human)

Tags

6 His

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-del C

Plasmid

#33095

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

deleted C terminal (amino acids 291-450)

Promoter

CMV/MSV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
TFORF3445

Plasmid

#144921

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PTTG1 (PTTG1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pmcherry α-catenin ΔVBS

Plasmid

#178649

Purpose

express α-catenin without a vinculin binding site (VBS)

Depositor

Daniel Conway

Article

Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

Insert

α-catenin (CTNNA1 Human)

Tags

mcherry

Expression

Mammalian

Mutation

VBS of alpha catenin was removed and replaced wit…

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2EmGFP-cDAXX

Plasmid

#169727

Purpose

Mammalian expression construct of GFP tagged cDAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

cDAXX (DAXX Human)

Tags

GFP

Expression

Mammalian

Mutation

Comprises residues 495-740

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-beta2(616-951)-mRuby2

Plasmid

#100730

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and mRuby2

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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TRIM28 gRNA (BRDN0001162440)

Plasmid

#76140

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM28

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM28 (TRIM28 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-FLIP pr5

Plasmid

#19130

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP promoter (CFLAR Human)

Use

Luciferase

Expression

Mammalian

Mutation

The sequence corresponds to positions -503 to +28…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2

Plasmid

#186361

Purpose

Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Human Ensconsin (MAP7 Human, Synthetic)

Use

Expression of a fluorescent microtubule marker

Tags

3xEGFP

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

Plasmid

#123338

Purpose

Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

DAKAP-Venus-cpVenus-FLARE-AKAR

Tags

N-terminal 30 amino acids from DAKAP1, Venus, and…

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-NCOA7g1 (BB22)

Plasmid

#139457

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-NCOA7g2 (BB23)

Plasmid

#139458

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-E2F1 3'UTR

Plasmid

#29468

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

E2F1 3'UTR (E2F1 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pET28-His8-aCat(660-906)

Plasmid

#203333

Purpose

Bacterial expression of alpha-catenin F-actin binding domain

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (CTNNA1 Human)

Tags

8xHis with precision protease cleavage site

Expression

Bacterial

Mutation

aa 660-906 only

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1395

Plasmid

#143106

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF569 (ZNF569 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero13

Plasmid

#187929

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero13

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero13 (BEX3 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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MTOR gRNA (BRDN0001147813)

Plasmid

#76783

Purpose

3rd generation lentiviral gRNA plasmid targeting human MTOR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MTOR gRNA (BRDN0001145082)

Plasmid

#76782

Purpose

3rd generation lentiviral gRNA plasmid targeting human MTOR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge