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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,313 results for: G3

Showing: 1301 - 1313 of 1313 results

  1. pXZ13lacZalpha

    Plasmid
    #160229
    Purpose
    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160229-plasmid-map-sequence-id-314764

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  2. CENTG3 (3IHW)

    Plasmid
    #25159
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CENTG3:P126-K291 (AGAP3 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains P126-K291
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pEf1a-Dn29-dCas9-P2A-GFP

    Plasmid
    #247155
    Purpose
    Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression casette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al bioRxiv 2024.11.01.621560
    Insert
    Dn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247155-plasmid-map-sequence-id-491690

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  6. pEf1a-goldDn29-dCas9-P2A-GFP

    Plasmid
    #247157
    Purpose
    Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression casette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al bioRxiv 2024.11.01.621560
    Insert
    goldDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247157-plasmid-map-sequence-id-491683

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  7. pEf1a-superDn29-dCas9-P2A-GFP

    Plasmid
    #247156
    Purpose
    Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression casette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al bioRxiv 2024.11.01.621560
    Insert
    superDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247156-plasmid-map-sequence-id-491689

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  8. pEf1a-hifiDn29-dCas9-P2A-GFP

    Plasmid
    #247158
    Purpose
    Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression casette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al bioRxiv 2024.11.01.621560
    Insert
    hifiDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247158-plasmid-map-sequence-id-491688

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  9. GPR97-DuET

    Plasmid
    #213294
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR97 (ADGRG3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213294-plasmid-map-sequence-id-430905

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  13. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Article
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    199344-plasmid-map-sequence-id-402934

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Showing: 1301 - 1313 of 1313 results