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pGY577

Plasmid

#166663

Purpose

Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Cre: E340A, D341A

Promoter

CMV

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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p2.1 (pAGL2A)

Plasmid

#27563

Depositor

Gregg Semenza

Article

Semenza et al J Biol Chem. 1996 Dec 20. 271(51):32529-

Insert

ENO1 promoter (ENO1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
pGEX4T2 RANK-L

Plasmid

#108571

Purpose

Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

Depositor

Jean-Claude Scimeca

Article

Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

Insert

RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

aa158-aa316

Promoter

Ptac (trp/lac)

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119B

Plasmid

#128333

Purpose

Mammalian expression vector of Myc-tagged human UNC119B

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119B (UNC119B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Plasmid

#180475

Purpose

Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

Zebrafish ubb promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGL2-Mcl-1

Plasmid

#19132

Depositor

Wafik El-Deiry

Article

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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p13xQUAS-ZsGreen-P2A, α-cry:mCherry

Plasmid

#180480

Purpose

Inducible gene expression vector p13xQUAS-ZsGreen-P2A, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

13x QUAS-ZsGreen-P2A

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-iCreV-4x6T

Plasmid

#196412

Purpose

Astrocytic expression of light-inducible iCreV recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

iCreV

Use

AAV; Astrocyte-selective

Tags

Expression

Mammalian

Mutation

Promoter

CAG and GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV EF1a DIO iChloC 2A dsRed

Plasmid

#70762

Purpose

An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)

Depositor

Thomas Oertner

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E83Q,E90R,E101S,D156N,T159C

Promoter

EF1a

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-ASAP2s

Plasmid

#101274

Purpose

Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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TOP2A sgRNA1

Plasmid

#138190

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A (TOP2A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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TOP2A sgRNA2

Plasmid

#138191

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A (TOP2A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

Plasmid

#176531

Purpose

PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

Depositor

Jared Toettcher

Article

Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

Inserts

KGV transcription factor
TagBFP

Use

Piggybac transposase integration vector

Tags

ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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TrHKI-pGFPN3

Plasmid

#21918

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Truncated human HKI (lacking exon 1 sequence that encodes for the mitochondrial binding domain) in pGFP-N3 plasmid (HK1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

This is the truncated human HKI cDNA (lacking exo…

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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AID-mTagBFP2-loxP_myo2_neoR-loxP

Plasmid

#194055

Purpose

Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

IAA17 (AXR3 Mustard Weed)

Use

Tags

codon optimized mTagBFP2

Expression

Worm

Mutation

Promoter

None

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSCALPS-mAgo2-EGFP

Plasmid

#209935

Purpose

Lentiviral expression of mouse Argonaute-2 in mammalian cells

Depositor

Phillip Zamore

Article

Jouravleva et al Cell Rep Methods. 2022 Mar 18;2(3):10018

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-GPD1

Plasmid

#32487

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1 (Gpd1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
mLama1 AAV sgRNA 1, 2, 5

Plasmid

#135339

Purpose

Expression plasmid of VP64-SadCas9 sgRNA 1, 2 and 3

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

Lama1 VP64-dCas9 sgRNA Guides

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad4

Plasmid

#89829

Purpose

Knockdown human Smad4 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

Smad4 shRNA (SMAD4 Mouse, Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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