We narrowed to 4,110 results for: LYC;

1. SYS1-2EGFP-ter-URA3

   Plasmid

   #184771

   Purpose

   Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   SYS1 (SYS1 Budding Yeast)
   

   Tags

   2EGFP

   Expression

   Yeast

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SEC7-2Katushka-ter-Hygromycin

   Plasmid

   #184772

   Purpose

   Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   SEC7 (SEC7 Budding Yeast)
   

   Tags

   2Katushka

   Expression

   Yeast

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PKC1-2GFP-ter-TRP1

   Plasmid

   #184773

   Purpose

   Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   PKC1 (PKC1 Budding Yeast)
   

   Tags

   2EGFP

   Expression

   Yeast

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-ammB3

   Plasmid

   #167815

   Purpose

   Expresses His6-tagged AmmB3 in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

   Insert

   AmmB3
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-ammC1

   Plasmid

   #167816

   Purpose

   Expresses His6-tagged AmmC1 in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

   Insert

   AmmC1
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAct-GST-H3(K27Q)

   Plasmid

   #182843

   Purpose

   a K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   GST-histone H3 fusion
   

   Tags

   GST

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAct-GST-H3

   Plasmid

   #182842

   Purpose

   A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   GST-histone H3 fusion
   

   Tags

   GST

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pb mD3S4-dN

   Plasmid

   #180252

   Purpose

   P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

   Depositor

   Timothy Springer

   Article

   Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

   Insert

   D3 of P. berghei HAP2 with N-glycosylation sites mutated
   

   Tags

   6His

   Expression

   Mammalian

   Mutation

   N516T, S533N and N539Q

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pThuAsol

   Plasmid

   #171675

   Purpose

   pThuAsol expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   SUMO-TEV-ThuAsol
   

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHCKan-T7-CBD-hGLY

    Plasmid

    #134940

    Purpose

    T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    human glycine acyltransferase 2 (GLYATL2 )
    

    Tags

    chitin binding domain

    Expression

    Bacterial

    Promoter

    t7

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pst39-NST-clone14

    Plasmid

    #133421

    Purpose

    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

    Depositor

    Gerhard Wagner

    Insert

    NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    ORF for Nip1 encodes aa 4-156 (not complete ORF),…

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-PC-1-CTS

    Plasmid

    #86067

    Purpose

    A bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 4297-4303

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. POLN delP_pDEST17

    Plasmid

    #64886

    Purpose

    bacterial expression of human POLN with a short truncation of the C-terminal proline-rich tail

    Depositor

    Richard Wood

    Article

    Takata et al J Biol Chem. 2006 Aug 18;281(33):23445-5

    Insert

    POLN (POLN Human)
    

    Tags

    Flag and His

    Expression

    Bacterial

    Mutation

    with a short truncation of the C-terminal proline…

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-NINJ1

    Plasmid

    #201180

    Purpose

    Transient expression of NINJ1 gene in mammalian cells

    Depositor

    Jonathan Kagan

    Article

    Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

    Insert

    Ninjurin 1 (Ninj1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDACR3882 [rab-3p::HYlight::let-858utr]

    Plasmid

    #207666

    Purpose

    Expresses worm-optimized HYlight in C. elegans neurons

    Depositor

    Daniel Colón-Ramos

    Article

    Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    HYlight Biosensor
    

    Expression

    Worm

    Promoter

    rab-3

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX puro TRIM21-GFP

    Plasmid

    #116941

    Purpose

    Expression of TRIM21-GFP fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 - GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. plenti hSyn PKM1

    Plasmid

    #220920

    Purpose

    Neuronal specific expression of PKM1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PKM1 (PKM Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGIPz-mPD-L1

    Plasmid

    #121488

    Purpose

    Expression of mouse PD-L1 WT

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRetroX-TRE3G_NINJ1

    Plasmid

    #201181

    Purpose

    Dox-inducible expression of NINJ1 gene in mammalian cells

    Depositor

    Jonathan Kagan

    Article

    Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

    Insert

    Ninjurin 1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Promoter

    TRE3G

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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