165,386 results

1. pCMV-dCas13-M3M14nes

   Plasmid

   #155367

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFPC1-hMyoX

   Plasmid

   #47608

   Depositor

   Emanuel Strehler

   Article

   Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

   Insert

   MyoX (MYO10 Human)
   

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Mutation

   See depositor comments below.

   Promoter

   CMV

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ203 (pMDC32-Ubi1)

   Plasmid

   #86207

   Purpose

   Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Type

   Empty backbone

   Use

   Gateway compatible attr1-attr2 destination vector

   Expression

   Plant

   Promoter

   Maize ubiquitin 1 (ZmUbi1)

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-EF1a MICA*009-IRES-ZsGreen

   Plasmid

   #114007

   Purpose

   Induces expression of human MICA allele 009 cDNA

   Depositor

   Kai Wucherpfennig

   Article

   Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

   Insert

   MICA*009 (MICA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_478

   Plasmid

   #179096

   Purpose

   BE Cas expression

   Depositor

   David Root, John Doench

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   BE3.9 (SpG)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

   Plasmid

   #126553

   Purpose

   Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

   Depositor

   Jonathan Karn

   Article

   Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

   Insert

   HIV-1 vector pNL4-3
   

   Use

   Lentiviral

   Tags

   Thy1.2 and GFP

   Promoter

   HIV LTR

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. OPRM1-Tango

   Plasmid

   #66464

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   OPRM1 (OPRM1 Human)
   

   Tags

   FLAG and tTA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVHA EED wt

   Plasmid

   #24231

   Purpose

   Mammalian expression of human EED with HA tag

   Depositor

   Kristian Helin

   Article

   Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

   Insert

   EED (EED Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS

   Plasmid

   #140008

   Purpose

   Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   4xPylT (Mma) cassette
   

   Expression

   Mammalian

   Mutation

   U25C

   Promoter

   h7SK

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDY1513 AAVS1 sgRNA

    Plasmid

    #234832

    Purpose

    sgRNA for AAVS1 STITCHR insertion

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    AAVS1 sgRNA
    

    Use

    CRISPR

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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