We narrowed to 24,431 results for: nes

1. pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

   Plasmid

   #218916

   Purpose

   Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

   Plasmid

   #187320

   Purpose

   dCas9 expression vector with cloning site for C terminal fusions

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

   Plasmid

   #60815

   Purpose

   dCas9 with single cysteine residue (M1C) for site-specific labelling

   Depositor

   Jennifer Doudna

   Article

   O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

   Insert

   dCas9 M1C D10A C80S H840A C574S
   

   Use

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   M1C D10A C80S H840A C574S

   Promoter

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. plx208-CMV-Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit

   Plasmid

   #213538

   Purpose

   Expresses the CapC-caged opioid peptide in mammalian cells

   Depositor

   Wenjing Wang

   Article

   Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

   Insert

   Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKG3658_pPV2-NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

   Plasmid

   #239699

   Purpose

   BsaI Golden Gate assembly plasmid encoding the COMET NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720 CDS

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV hU6-Non-target sgRNA Nestin-dCas9-KRAB-T2a-GFP

   Plasmid

   #196989

   Purpose

   Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with non-target sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

   Insert

   Non-Target
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

   Plasmid

   #228399

   Purpose

   Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasm

   Depositor

   Dirk Daelemans

   Article

   Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

   Insert

   3xFLAG-NLS/SV40-AcGFP-NES/PKI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

   Plasmid

   #210739

   Purpose

   Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   Sp Cas9 (cas9 S pyogenes)
   Sp sgRNA GFP Target 3
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   Promoter

   H1 and eCMV

   Available since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-ITR2-H1-GFP sgRNA Target 1- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

   Plasmid

   #210737

   Purpose

   Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 1

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   Sp Cas9 (cas9 S pyogenes)
   Sp sgRNA GFP Target 1
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   Promoter

   H1 and eCMV

   Available since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-ITR2-H1-GFP sgRNA Target 2- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210738

    Purpose

    Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 2

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    Sp Cas9 (cas9 S pyogenes)
    Sp sgRNA GFP Target 2
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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