We narrowed to 2,414 results for: organization Addgene

1. pVC-DNAJB6b

   Plasmid

   #175065

   Purpose

   expresses mVenus and mCherry tagged DNAJB6b in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   DNAJB6b (DNAJB6 Human)
   

   Use

   Tags

   V5 and mVenus + mCherry

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-DYRK1A-2A-mCherry

   Plasmid

   #118272

   Purpose

   Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
   

   Use

   AAV

   Tags

   2A peptide and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV with beta global intron

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

   Plasmid

   #140163

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. XIAP (pBS0852)

   Plasmid

   #185282

   Purpose

   For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   XIAP (XIAP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2PH-PLCd-APEX2-P2A-mKate2

   Plasmid

   #71284

   Purpose

   Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   2PH-PLCd-APEX2-P2A-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST_DSUP_RAMVA (pBS0353)

   Plasmid

   #185189

   Purpose

   For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DSUP_RAMVA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HER3-DMVF-181-R

   Plasmid

   #87621

   Purpose

   C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   HER3-DMVF181-C (ERBB3 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Venus-173-N-ORAI1

   Plasmid

   #87618

   Purpose

   N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   Venus173-N-ORAI1 (ORAI1 Synthetic, Human)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. miniSOG-Golgi-7

   Plasmid

   #57768

   Purpose

   Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Golgi (B4GALT1 Human)
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   aa 1-82 of NM_001497.3

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAAV-DCX(S306D)-2A-mCherry

    Plasmid

    #118292

    Purpose

    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    Doublecortin (DCX Human)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    Serine 306 changed to Aspartic Acid

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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