We narrowed to 5,252 results for: pcDNA3.1+

1. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

   Plasmid

   #159129

   Purpose

   expresses NRF2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-VEGFR2-mCherry

   Plasmid

   #139606

   Purpose

   Expresses a VEGFR2-mCherry fusion protein in mammalian cells

   Depositor

   Michael Welsh

   Article

   Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1+FLAG-KrascomG12D

   Plasmid

   #206844

   Purpose

   To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codons altered to most optimum based on mouse cod…

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

   Plasmid

   #187729

   Purpose

   Express the catalytically inactive NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Cys874 mutated to a serine

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-2xFLAG-SREBP-2

   Plasmid

   #26807

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   L7V, G76S, K395R and I482L

   Promoter

   CMV

   Available since

   July 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-FGFR2c-V5/HIS

   Plasmid

   #201107

   Purpose

   expression of human FGFR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)Gαi3-91-NLuc

   Plasmid

   #207150

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAI3 (GNAI3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)K

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-Elongin B-pcDNA3.1(+)-Zeo

   Plasmid

   #20000

   Depositor

   William Kaelin

   Article

   Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

   Insert

   transcription elongation factor B (ELOB Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-ccdB-3xFLAG

    Plasmid

    #214984

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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