We narrowed to 7,484 results for: PAC

1. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut_R

   Plasmid

   #147388

   Purpose

   Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut (gw Nematode, Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A_R

   Plasmid

   #147391

   Purpose

   Insect Expression of CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut-CIM2mut_R

   Plasmid

   #147396

   Purpose

   Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut (TNRC6C Nematode, Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-mut_R

   Plasmid

   #147385

   Purpose

   Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-mut

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-DmGW182_635-1384-CIM1-mut (gw Nematode, Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

   Plasmid

   #228788

   Purpose

   EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   EGFP***
   TEV protease
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

   Plasmid

   #172501

   Purpose

   Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 full locus with Golden gate acceptor site for spacer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

   Plasmid

   #202719

   Purpose

   Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Myr-Pink Flamindo
   EGFP-bPAC-CAAX
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

   Plasmid

   #52890

   Purpose

   "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

   Depositor

   Iswar Hariharan

   Article

   Bosch et al Development. 2015 Feb 1;142(3):597-606.

   Inserts

   LexGAD
   Gal4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

   Plasmid

   #176588

   Purpose

   Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Delaware Bay) locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Replaced spacer in CRISPR array with Fn spacer

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228787

    Purpose

    EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1-PH-mCherry-Oskar 139-606 isoform A (Short Oskar) (HK195)

    Plasmid

    #206460

    Purpose

    Oskar 139-606 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Oskar 139-606 (osk Fly)
    

    Use

    Tags

    PH mcherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1-PH-mCherry-Oskar 139-240 MUT (A162E/L228E) (HK202)

    Plasmid

    #206464

    Purpose

    Oskar 139-240 mut expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Oskar 139-240 mut (osk Fly)
    

    Use

    Tags

    PH mcherry

    Expression

    Insect

    Mutation

    A162E L228E)

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-lambdaN-HA-GST-DmRoquin_702-819-del725-755-del790-812_AE

    Plasmid

    #148606

    Purpose

    Insect Expression of DmRoquin_702-819-del725-755-del790-812

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin_702-819-del725-755-del790-812 (roq Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A_S

    Plasmid

    #147515

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A (gw Nematode, Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A_R

    Plasmid

    #147389

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A (gw Nematode, Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R

    Plasmid

    #147394

    Purpose

    Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Nematode, Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid

    #197031

    Purpose

    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized denOsCas12f1-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D52R / T132R / D228A / D406A

    Promoter

    CBh, CMV, hU6

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7

    Plasmid

    #214140

    Purpose

    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Use

    Tags

    2xSTII

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid

    #214139

    Purpose

    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Use

    Tags

    2xSTII

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid

    #101230

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Plasmid

    #101231

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…

    Promoter

    T7

    Available since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Plasmid

    #101229

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

    Plasmid

    #189635

    Purpose

    Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AiP14002 - pAAV-AiE0064h-spacer-AiE0022h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4002)

    Plasmid

    #230389

    Purpose

    AiE0064h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EW375 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid

    #236177

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and GFP-NZF dummy transcription factor

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    EGFP-NZF
    H2B (H2BC11 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and four ZNF35/IKZF1 sites

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EW373 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid

    #236175

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and GFP-NZF dummy transcription factor

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    EGFP-NZF
    H2B (H2BC11 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and four ZNF35/ZNF250 sites

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EW374 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid

    #236176

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and GFP-NZF dummy transcription factor

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    EGFP-NZF
    H2B (H2BC11 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and four ZNF35/ZNF35 sites

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid

    #176459

    Purpose

    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid

    #176460

    Purpose

    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EW371 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deimlink-NZF

    Plasmid

    #236173

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and ZNF35/ZNF35-NZF transcription factor

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF
    H2B (H2BC11 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and four ZNF35/ZNF35 sites

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EW370 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deimlink-NZF

    Plasmid

    #236172

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and ZNF35/ZNF250-NZF transcription factor

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
    H2B (H2BC11 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and four ZNF35/ZNF250 sites

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EW372 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-IKZF1(2-4)-deimlink-NZF

    Plasmid

    #236174

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and ZNF35/IKZF1-NZF transcription factor (with the IKZF1 portion containing ZFs 2-4 from that protein)

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    ZNF35/IKZF1(2-4)-deimmunLink-NZF
    H2B (H2BC11 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and four ZNF35/IKZF1 sites

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. NK676

    Plasmid

    #120852

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Insert

    pac-P2A-mTFP
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. NK696

    Plasmid

    #120855

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Insert

    pac-P2A-mTFP-Jumble
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NK694

    Plasmid

    #120853

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Insert

    pac-P2A-Jumble-mTFP
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GLYR1

    Plasmid

    #38946

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GLYR1 (GLYR1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pTR(EF)-tRNA(Q)

    Plasmid

    #170517

    Purpose

    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(EF)-tRNA(Q)
    

    Use

    Pcr template vector for amplifying grnacore(ef)-t…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. NK697

    Plasmid

    #120856

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Insert

    pac-P2A-mTFP-Jumblelw1
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Jumblelw1 allele: T to C at nt 1109 causes L305P …

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. NK695

    Plasmid

    #120854

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Insert

    pac-P2A-Jumblelw1-mTFP
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Jumblelw1 allele: T to C at nt 1109 causes L305P …

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMS18

    Plasmid

    #225681

    Purpose

    Confers puromycin resistance to Salpingoeca rosetta

    Depositor

    Thibaut Brunet

    Article

    Combredet et al bioRxiv 2024.07.13.603360

    Insert

    pEFL-Pac-Actin_3'UTR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge