We narrowed to 14,584 results for: pET

1. pET21d-colEdes2/Imdes2

   Plasmid

   #107109

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des2

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes2/Imdes2
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21d-colEdes3/Imdes3

   Plasmid

   #107110

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes3/Imdes3
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21d-colEdes3.5/Imdes3.5

   Plasmid

   #107111

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes3.5/Imdes3.5
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21d-colEdes4/Imdes4

   Plasmid

   #107112

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes4/Imdes4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21d-colEdes5/Imdes5

   Plasmid

   #107113

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes5/Imdes5
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21d-colEdes6/Imdes6

   Plasmid

   #107114

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes6/Imdes6
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFS_0387_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2

   Plasmid

   #116999

   Purpose

   Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Rivularia sp. PCC 7116 RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFS_0388_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2_RC

   Plasmid

   #117000

   Purpose

   Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Rivularia sp. PCC 7116 RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFS_0386_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1_RC

   Plasmid

   #116998

   Purpose

   Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Rivularia sp. PCC 7116 RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFS_0347_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1

    Plasmid

    #116959

    Purpose

    Expresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Campylobacter fetus subsp. Fetus RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFS_0348_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1_RC

    Plasmid

    #116960

    Purpose

    Expresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1 RC, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Campylobacter fetus subsp. Fetus RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET51b(+)_MBP-nLucCP-65/68

    Plasmid

    #113921

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-65/68
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET51b(+)_MBP-nLucCP-65/66

    Plasmid

    #113920

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-65/66
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET51b(+)-LUCIDRS-MTX

    Plasmid

    #113925

    Purpose

    Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic biosensor for methotrexate

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    LUCID_RS-MTX
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET29b-clbI S178A

    Plasmid

    #114159

    Purpose

    Expresses ClbI S178A

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbI
    

    Use

    Tags

    6xHis and 6xHis-Thrombin site

    Expression

    Bacterial

    Mutation

    Mutated S178 to Ala

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-clbH-C-A2-PCP

    Plasmid

    #114157

    Purpose

    Expresses ClbH-C-A2-PCP

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbH
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Only contains the C-A2-PCP domains of ClbH

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_6HT-TBP6.7(P46A)

    Plasmid

    #112721

    Purpose

    This mutation (P46A) marks the first residue within the evolved region of U1A.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P46A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P46A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a_6HT-TBP6.7(R47K)

    Plasmid

    #112723

    Purpose

    Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47K)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47K

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a_6HT-TBP6.7(Q48A)

    Plasmid

    #112724

    Purpose

    Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a_6HT-TBP6.7(Q48T)

    Plasmid

    #112725

    Purpose

    This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48T)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48T

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a_6HT-TBP6.7(T50A)

    Plasmid

    #112727

    Purpose

    Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(T50A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    T50A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a_6HT-TBP6.7(P51A)

    Plasmid

    #112728

    Purpose

    Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P51A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P51A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a_6HT-TBP6.7(R52A)

    Plasmid

    #112729

    Purpose

    52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R52A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R52A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a_6HT-TBP6.7(Q54A)

    Plasmid

    #112730

    Purpose

    Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q54A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q54A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-MBP-CreM_E352A

    Plasmid

    #112239

    Purpose

    pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM with E352A mutation

    Depositor

    Emily Balskus

    Article

    Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

    Insert

    CreM_E352A
    

    Use

    Tags

    MBP

    Expression

    Mutation

    Glutamate 352 (E352) mutated to an alanine.

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-30a(+)-C-SH2

    Plasmid

    #111419

    Purpose

    express murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

    Insert

    murine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-30a(+)-C-SH2+IA

    Plasmid

    #111418

    Purpose

    express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

    Insert

    murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-30a(+)-N-SH2

    Plasmid

    #111274

    Purpose

    express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    

    Use

    Tags

    HHHHHHENLYFQG

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-30a(+)-N-SH2+IA

    Plasmid

    #111273

    Purpose

    express murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    

    Use

    Tags

    HHHHHHENLYFQG

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a-RupA_T1-NHis

    Plasmid

    #109259

    Purpose

    NRPS T domain with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_T1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    contains aa 917..1025 of WP_015523502.1

    Promoter

    T7

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET29b-RupA_T2-R-CHis

    Plasmid

    #109258

    Purpose

    NRPS di-domain containing TR domains with C-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_T2-R
    

    Use

    Tags

    His tag and S-Tag

    Expression

    Bacterial

    Mutation

    contains aa 1939..2443 of WP_015523502.1

    Promoter

    T7

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-RupA_R-NHis

    Plasmid

    #109257

    Purpose

    NRPS R domain with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_R
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    contains aa 2038..2443 of WP_015523502.1

    Promoter

    T7

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET29b-RupA_C2-A2-T2-R-CHis

    Plasmid

    #109256

    Purpose

    NRPS module containing CATR domains with C-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_C2-A2-T2-R
    

    Use

    Tags

    His tag and S-Tag

    Expression

    Bacterial

    Mutation

    contains aa 1023..2443 of WP_015523502.1

    Promoter

    T7

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET29b-RupA_C1-A1-T1-CHis

    Plasmid

    #109255

    Purpose

    NRPS module containing CAT domains with C-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_C1-A1-T1
    

    Use

    Tags

    His tag and S-Tag

    Expression

    Bacterial

    Mutation

    contains aa 1..1025 of WP_015523502.1

    Promoter

    T7

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET21a - Ec-ClpX D144L

    Plasmid

    #111514

    Purpose

    Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    ClpX
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Aspartatic acid 144 to Leucine

    Promoter

    T7

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET24a-VHH-1xTS

    Plasmid

    #109418

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET22b-ecDHFR-dCys

    Plasmid

    #110541

    Purpose

    Bacterial protein of E.coli DHFR C85A/C152S mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C85 mutated to A85, C152 mutated to S152

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET27b-ecDHFR-N23PP

    Plasmid

    #110828

    Purpose

    Bacterial expression of E.coli DHFR N23PP mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28b+R3(R112C/T180C)C

    Plasmid

    #108194

    Purpose

    Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C)-P450cam fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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