We narrowed to 1,479 results for: pbluescript

1. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

   Plasmid

   #184398

   Purpose

   Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase beta-globin-PTC39
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   PTC in beta-globin at amino acid position 39

   Promoter

   Tet-On 3G

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMW35 p21 (mouse)

   Plasmid

   #16478

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

   Insert

   p21 (Cdkn1a Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pT2-7xTcf-NLS-CNL

   Plasmid

   #65712

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, CNL
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

   Plasmid

   #101349

   Purpose

   2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta tat delta TAR delta env GFP in Nef Tet ope…

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMCS.71/2272.Hygro

   Plasmid

   #22731

   Depositor

   Mark Magnuson

   Article

   Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

   Insert

   Lox71-FRT-PGK-hygro-FRT-Lox2272
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   This is a basal exchange vector for RMCE. The vec…

   Promoter

   Available since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DMP1-GFPtopaz

   Plasmid

   #114270

   Purpose

   GFP labeling of mature osteoblasts and osteocytes.

   Depositor

   David Rowe

   Article

   Kalajzic et al Bone. 2004 Jul;35(1):74-82. doi: 10.1016

   Insert

   Dmp1 + GFPtopaz (Dmp1 Mouse, Aequorea victoria)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dmp1

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lox-stop-Lox p53 R270H

   Plasmid

   #14853

   Depositor

   Tyler Jacks

   Article

   Olive et al Cell. 2004 Dec 17. 119(6):847-60.

   Insert

   p53 R270H (Trp53 Mouse)
   

   Use

   Cre/Lox

   Tags

   Lox-STOP-lox

   Expression

   Mammalian

   Mutation

   Contact mutant R270H. (Murine p53 codon 270 corre…

   Promoter

   Available since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

8. PG13-gal (wt p53 binding sites)

   Plasmid

   #16446

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   p53-binding site (TP53 )
   

   Use

   Beta-gal reporter

   Tags

   beta-gal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

9. Neurod1-Atoh7HA3

   Plasmid

   #44438

   Depositor

   William Klein

   Article

   Mao et al Development. 2013 Feb;140(3):541-51. doi

   Insert

   Atoh7-3xHA knock-in (Atoh7 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Neurod1 left arm, Neurod1 right arm, and loxC2-Re…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MG15-gal (mut p53 binding sites)

    Plasmid

    #16447

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    p53-binding site (mutant) (TP53 )
    

    Use

    Beta-gal reporter

    Tags

    beta-gal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pBS NL4-3 envFS eGFP 5'CMV Δtat

    Plasmid

    #101347

    Purpose

    tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta Tat

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-AMV-α1L9'S-nAchR

    Plasmid

    #20684

    Depositor

    Henry Lester

    Article

    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

    Insert

    nicotinic acetylcholine receptors (Chrna1 Mouse)
    

    Use

    Tags

    AMV and pA 50

    Expression

    Bacterial

    Mutation

    Leucine at 9' position in M2 region changed …

    Promoter

    Available since

    April 3, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pBSK-SN3 (wt p53)

    Plasmid

    #16421

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1989 Apr 14. 244(4901):217-21.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

15. D3 clone (p21/WAF1 promoter)

    Plasmid

    #16452

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    WAF1 promoter (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pDP1.2

    Plasmid

    #171092

    Purpose

    Expresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    AAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
    

    Use

    Synthetic Biology

    Tags

    Emerald, Geminin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS-Squ5'3'

    Plasmid

    #20165

    Depositor

    Eric Wieschaus

    Article

    Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

    Insert

    spaghetti squash (squ, myosin II RLC) 5' UTR, ORF (without stop codon) and 3' UTR with multiple cloning site downstream (sqh Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pBS-tRNA-Ser

    Plasmid

    #174318

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-ser
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBSSK p85 alpha (delta SH3, iSH2)

    Plasmid

    #13433

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta SH3, iSH2) (PIK3R1 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Flag tags replacing both the SH3 and iSH2 domains.

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pCA-G-intron(Neo)-T(FLLFLNL)

    Plasmid

    #40020

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdT-3Myc
    beta-globin intron
    Neo
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge