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We narrowed to 6,946 results for: pcDNA3.1+

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Showing: 1321 - 1340 of 6946 results

  2. pcDNA3.1-CHRFAM7A-mCherry

    Plasmid
    #62635
    Purpose
    mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop
    Depositor
    Henry Lester
    Publication
    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHRFAM7A (CHRFAM7A Human)
    Expression
    Mammalian
    Mutation
    mCherry inserted into M3-M4 loop
    Promoter
    CMV
    Available since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles

  6. pcDNA3.1(+)-ExRai-AKAR-NES

    Plasmid
    #118407
    Purpose
    Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AKAR-NES
    Tags
    Nuclear export signal (NES)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118407-plasmid-map-sequence-id-230143

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  7. FLAG-OTUB1 C91S-pcDNA3.1

    Plasmid
    #118210
    Purpose
    Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells
    Depositor
    Cynthia Wolberger
    Publication
    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OTUB1 (OTUB1 Human)
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    changed cysteine 91 to serine
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    118210-plasmid-map-sequence-id-226713

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  8. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Publication
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCV2
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112060-plasmid-map-sequence-id-216934

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  9. mTiam1-mcherry-sspB in pcDNA3.1

    Plasmid
    #85221
    Purpose
    Role of Rac selective GEF domain from Tiam1 in immune cell migration
    Depositor
    Narasimhan Gautam
    Publication
    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mTiam1 (1030-1406) (Tiam1 Mouse)
    mcherry
    sspB
    Tags
    none
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    85221-plasmid-map-sequence-id-169613

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  13. pcDNA3.1-HA-Bax

    Plasmid
    #191944
    Purpose
    Expression of HA-Bax.
    Depositor
    Luca Scorrano
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HA-Bax (Bax Mouse)
    Expression
    Mammalian
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191944-plasmid-map-sequence-id-379568

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  16. pcDNA3.1-N-Luc

    Plasmid
    #185357
    Purpose
    For mammalian expression of N-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 2-416).
    Depositor
    Ayaz Najafov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Firefly Luciferase
    Expression
    Mammalian
    Mutation
    WT
    Available since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185357-plasmid-map-sequence-id-363972

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  18. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid
    #118465
    Purpose
    Negative-control mutant for sapphireAKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sapphireAKAR(T/A)
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    118465-plasmid-map-sequence-id-230227

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  19. pcDNA3.1(+)-ER-BSCaM2

    Plasmid
    #64855
    Purpose
    Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CYP450-BSCaM2
    Tags
    CFP, N-terminal 27 amino acids from CYP450, and Y…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64855-plasmid-map-sequence-id-109681

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  20. pcDNA3.1-CHRFAM7ADelta2bp

    Plasmid
    #62515
    Purpose
    mammalian expression of human duplicated Alpha7 with 2 bp deletion (CHRFAM7A-∆2bp)
    Depositor
    Sherry Leonard, Henry Lester
    Publication
    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHRFAM7ADelta2bp (CHRFAM7A Human)
    Expression
    Mammalian
    Mutation
    partially duplication of CHRNA7 with 2-bp deletio…
    Promoter
    CMV
    Available since
    March 20, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1321 - 1340 of 6946 results