We narrowed to 1,373 results for: tox
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Plasmid#73189PurposeTemplate plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertchloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
UseTagsExpressionBacterialMutationPromoterchloramphenicol promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJR019
Plasmid#198834PurposeInactivation of G protein subunits through Pertussis toxin (PTX)DepositorInsert15xUAS::PTX-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-GT
Plasmid#40025DepositorInsertsGFP
beta-globin intron
Neo
tdT-3Myc
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-TG
Plasmid#40026DepositorInsertsT (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A
UseTagsExpressionMammalianMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJH4504
Plasmid#191702PurposePnmr-1 long::TeTx::wCherry unc-54 3' UTR C.elegans AVA/other neurons expression of TeTx RFPDepositorInsertTetanus Toxin Light Chain
UseTagswCherryExpressionWormMutationPromoterPnmr-1 longAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
UseTagsExpressionBacterialMutationPromoterlacUV5Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
JBEI-3716
Plasmid#100169PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertcodon-optimized MK
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTG8-Amp-FRT
Plasmid#128277PurposeTIP-LITer2.0-KRAS(G12V) gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFPDepositorInsertsTetR-LOV-TIP
EGFP
KRAS(G12V)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rac1(H40,L61)
Plasmid#11403DepositorInsertras-related C3 botulinum toxin substrate 1 (RAC1 Human)
UseTagsYFPExpressionMammalianMutationObtained with Glutamine 61 mutated to Leucine. Th…PromoterAvailable sinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
mCit-Snap25b D179K A199R
Plasmid#53259PurposeSNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationD179K and A199RPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS exon37a pCAGGS
Plasmid#206086Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37aDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR017
Plasmid#198835PurposeIn vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.DepositorInsert15xUAS::TeTX-SL2-mKate::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW491
Plasmid#198808PurposeIn vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.DepositorInsert15xUAS::Tetx cDNA::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH2633
Plasmid#192107PurposePsra-11-TeTx::wCherry unc-54 3' UTR C.elegans AVB and other neurons expression of TeTx RFPDepositorInsertTetanus Toxin Light Chain
UseTagswCherryExpressionWormMutationPromoterPsra-11Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308/pET21a(+)
Plasmid#83293Purposecsd2, construct: 121-308, pET21a(+) no-tagDepositorInserthp1544
UseTagsExpressionBacterialMutationdeleted amino acid 1-120PromoterAvailable sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_140-251/pET21a(+)
Plasmid#83294Purposecsd2, construct:140-251, pET21a(+) C-tagDepositorInserthp1544
UseTagsHis tagExpressionBacterialMutationdeleted amino acids 1-139 and 252-308PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd1_54-312/pET21a(+)
Plasmid#83291Purposecsd1, construct: 54-312, pET21a(+) C-tagDepositorInserthp1543
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-53PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308/pET28b(+)
Plasmid#83292Purposecsd2, construct: 121-308, pET28b(+) NC-tagDepositorInserthp1544
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-120PromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd1_125-312/pET28b(+)
Plasmid#83290Purposecsd1, construct: 125-312, pET28b(+) NC-tagDepositorInserthp1543
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-124PromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterNative promoter from pMUT2 relB/relE operon and P…Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only