165,528 results

1. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

   Plasmid

   #183409

   Purpose

   piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3_QLuc

   Plasmid

   #183260

   Purpose

   Express QLuc in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

   Insert

   QLuc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSIIbleo-iRFP670-P2A-EGFP

   Plasmid

   #198060

   Purpose

   The lentiviral vector for iRFP670 and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   iRFP670
   

   Use

   Lentiviral

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100854

   Purpose

   AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

   Insert

   jRGECO1a
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

   Plasmid

   #209078

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   3K mutant: E35K, E46K, E61K

   Promoter

   TRE4G

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-CMV-TetO2_3C-Twin-Strep

   Plasmid

   #113883

   Purpose

   Mammalian (inducible) protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-IFT20-EGFP

   Plasmid

   #118032

   Purpose

   Expresses IFT20-EGFP in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Insert

   human IFT20 (IFT20 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   EF1 alpha

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-mROCK2

   Plasmid

   #101296

   Purpose

   Mammalian expression of Rock2-GFP

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock2 (Rock2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57.1 blast tBID-flag

   Plasmid

   #211533

   Purpose

   Expresses truncated BID upon doxycycline addition to trigger apoptosis

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   tBID-flag (BID Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Truncated (CASP8) form (p15)

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGEN-dPspCas13b-NES-mCherry

    Plasmid

    #233609

    Purpose

    Expression of catalytically-inactive PspCas13b in mammalian cells

    Depositor

    Shintaro Iwasaki

    Article

    Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

    Insert

    dPspCas13b-NES-mCherry
    

    Use

    CRISPR

    Tags

    3xFlag and mCherry

    Mutation

    H133A/H1058A

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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