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Plasmid#208872PurposeFor expression in E. coli of NAP1 (delta-NDP52)DepositorInsertNAP1 (AZI2 Human)
UseTagsMBP-TEVExpressionBacterialMutationS37K/A44E (disrupts NDP52 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)
Plasmid#208873PurposeFor expression in E. coli of NAP1 (delta-TBK1)DepositorInsertNAP1 (AZI2 Human)
UseTagsMBP-TEVExpressionBacterialMutationL226Q/L233Q (disrupts TBK1 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving
Plasmid#204811PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCIneo-V5-SBP-MBP-HsNot4_64-104-GSSG-275-575_AG
Plasmid#148765PurposeMammalian Expression of HsNot4_64-104-GSSG_275-575DepositorInsertHsNot4_64-104-GSSG_275-575 (CNOT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-MBP-HsNot4_109-189-GSSG-275-575_AG
Plasmid#148767PurposeMammalian Expression of HsNot4_109-189-GSSG_275-575DepositorInsertHsNot4_109-189-GSSG_275-575 (CNOT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP
Plasmid#190039PurposeExpression of FIP200 mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsGST and MBPExpressionMammalianMutation(641-779)deletion & 1363 RDKDLIES 1370 to GSSā¦PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
Plasmid#177849PurposeBacterial Expression of PNRDepositorInsertphotoreceptor cell-specific nuclear receptor (NR2E3 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r
Plasmid#162950PurposeGateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed proteinDepositorInsertdual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r
Plasmid#162964PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r
Plasmid#162957PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only