We narrowed to 2,368 results for: pcas9

1. pL-CRISPR.EFS.PAC

   Plasmid

   #57828

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

   Plasmid

   #135014

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   126aa domain from HSV-1 UL12 fused to the C-termi…

   Promoter

   CBh

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

   Plasmid

   #135013

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   126aa domain from HSV-1 UL12 fused to the N-termi…

   Promoter

   CBh

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. L40C-CRISPR.EFS.mNeon

   Plasmid

   #69146

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PEmax-P2A-hP53DD

   Plasmid

   #214084

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PEmax-P2A-hP53DD (TP53 SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus, Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458-gR2A_ISO

   Plasmid

   #124808

   Purpose

   Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   hSPCas9
   

   Use

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. nCas9-UGI control for Target-AID (pRZ1047)

   Plasmid

   #131299

   Purpose

   CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNA_Cloning Vector BbsI

   Plasmid

   #128433

   Purpose

   Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing

   Depositor

   Izuho Hatada

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9 NGFR

   Plasmid

   #155279

   Purpose

   Lentiviral Cas9 expression with NGFR marker

   Depositor

   Martin Carroll

   Insert

   spCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas9 CFP

    Plasmid

    #155278

    Purpose

    Lentiviral Cas9 expression with CFP marker

    Depositor

    Martin Carroll

    Insert

    spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti Cas9/SB100

    Plasmid

    #155294

    Purpose

    Lentiviral expression of Cas9 and SB100

    Depositor

    Martin Carroll

    Insert

    SB100X-P2A-spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas9 ROSA sgRNA mCherry

    Plasmid

    #155280

    Purpose

    Lentiviral Cas9 expression, ROSA sgRNA expression, mCherry marker

    Depositor

    Martin Carroll

    Insert

    spCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK1520_blastR

    Plasmid

    #175289

    Purpose

    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection

    Depositor

    Ronald Cohn

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/U6

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBACH2.2.0-gDNA

    Plasmid

    #176375

    Purpose

    Expresses spCas9 and gRNA sequence targeting BACH2

    Depositor

    Barbara Stranger, Kevin White

    Insert

    BACH2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSOX2.1.0-gDNA

    Plasmid

    #176382

    Purpose

    Expresses spCas9 and gRNA sequence targeting SOX2

    Depositor

    Barbara Stranger, Kevin White

    Insert

    SOX2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti.Cas9.BFP.sgRNA.parental

    Plasmid

    #196715

    Purpose

    Sortable WT-SpCas9 expression and guide cassette (1-vector system).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSOX11.1.0-gDNA

    Plasmid

    #176378

    Purpose

    Expresses spCas9 and gRNA sequence targeting SOX11

    Depositor

    Barbara Stranger, Kevin White

    Insert

    SOX11 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRERF1.1.0-gDNA

    Plasmid

    #176384

    Purpose

    Expresses spCas9 and gRNA sequence targeting TRERF1

    Depositor

    Barbara Stranger, Kevin White

    Insert

    TRERF1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZNF627.1.0-gDNA

    Plasmid

    #176383

    Purpose

    Expresses spCas9 and gRNA sequence targeting ZNF627

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZNF627 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOSR1.1.0-gDNA

    Plasmid

    #176381

    Purpose

    Expresses spCas9 and gRNA sequence targeting OSR1

    Depositor

    Barbara Stranger, Kevin White

    Insert

    OSR1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge