We narrowed to 8,806 results for: ef1;

1. pGBW-m4046564

   Plasmid

   #145432

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGBW-m4046591

   Plasmid

   #145437

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF7b

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046360

   Plasmid

   #145414

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4046169

   Plasmid

   #145411

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 endoRNAse

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 endoRNAse (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pGBW-m4046471

   Plasmid

   #145398

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 endoRNAse

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046266

   Plasmid

   #145394

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046168

   Plasmid

   #145382

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF7b

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4046544

   Plasmid

   #145408

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046358

   Plasmid

   #145396

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 endoRNAse

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 endoRNAse (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4046466

    Plasmid

    #145466

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pPEE20

    Plasmid

    #128363

    Purpose

    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the amdS2 counterselectable marker.

    Depositor

    James Fraser

    Article

    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPEE16

    Plasmid

    #128360

    Purpose

    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 1, a small gene-free region. Contains the amdS2 counterselectable marker.

    Depositor

    James Fraser

    Article

    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPEE22

    Plasmid

    #128365

    Purpose

    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the amdS2 counterselectable marker.

    Depositor

    James Fraser

    Article

    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPEE21

    Plasmid

    #128364

    Purpose

    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the amdS2 counterselectable marker.

    Depositor

    James Fraser

    Article

    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPEE23

    Plasmid

    #128366

    Purpose

    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the amdS2 counterselectable marker.

    Depositor

    James Fraser

    Article

    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPEE19

    Plasmid

    #128362

    Purpose

    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the amdS2 counterselectable marker.

    Depositor

    James Fraser

    Article

    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKER-Clover

    Plasmid

    #85974

    Purpose

    Expresses Clover from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    Clover
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef-1a

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDS3-X4

    Plasmid

    #70140

    Purpose

    Yeast chr. integration vector with DS3 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    DS3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AgTEF1

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSBbi-Pur

    Plasmid

    #60523

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYSEAP

    Plasmid

    #37326

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PB-TO-MYOD1-shOct4

    Plasmid

    #182309

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    shOct4

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSBbi-Neo

    Plasmid

    #60525

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSBbi-Hyg

    Plasmid

    #60524

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: hygromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSBbi-RN

    Plasmid

    #60519

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSBbi-Bla

    Plasmid

    #60526

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLARRY-EGFP

    Plasmid

    #140025

    Purpose

    Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

    Depositor

    Fernando Camargo

    Article

    Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSBbi-GN

    Plasmid

    #60517

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. WT FL USP7

    Plasmid

    #227214

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7-promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

    Plasmid

    #219536

    Purpose

    a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204723

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSLQ11438

    Plasmid

    #183204

    Purpose

    pHR-EF1a-ABE8e-hyperdLbCas12a-NLS-mCherry-P2A-Puro-WPRE

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    hyperdCas12 base editor
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Nampt-PET28a

    Plasmid

    #25630

    Depositor

    Cynthia Wolberger

    Article

    Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):66

    Insert

    Nampt (Nampt Mouse)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

33. pSBbi-BN

    Plasmid

    #60518

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAV-hACE2-cMYC-Flag

    Plasmid

    #158957

    Purpose

    Used for expression of human ACE2 with AAV packaging

    Depositor

    Akiko Iwasaki

    Insert

    Human ACE2 (ACE2 Human)
    

    Use

    AAV

    Tags

    Flag and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid

    #164637

    Purpose

    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

    Depositor

    Günter Schneider

    Insert

    target guide sequence PRMT5 inhibition (PRMT5 Human)
    

    Use

    CRISPR and Lentiviral; Interference

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p413TEF-Sir2

    Plasmid

    #51742

    Purpose

    overexpression of Sir2 in S. cerevisiae, HIS3 marker

    Depositor

    Markus Ralser

    Article

    Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

    Insert

    Sir2 (SIR2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-AP.Adamts2.1-GFP-WPRE

    Plasmid

    #211488

    Purpose

    expresses GFP under artificial promoter Adamts2

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV-EgI-BlastR

    Plasmid

    #207176

    Purpose

    Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-BlastR

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. ptwB

    Plasmid

    #48735

    Purpose

    Tandem dual tomato plasmid

    Depositor

    John Schiller, Roger Tsien

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    Tandem dual tomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGBW-m4046233

    Plasmid

    #149032

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pGBW-m4046664

    Plasmid

    #149224

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pLV-EgI-NeoR

    Plasmid

    #207175

    Purpose

    Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoR

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. aCE120

    Plasmid

    #204491

    Purpose

    Figure 1. reporter circuit

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    EBFP2
    mKate2
    EYFP
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLOX-EWgfp

    Plasmid

    #12242

    Depositor

    Didier Trono

    Article

    Salmon et al Blood. 2000 Nov 15. 96(10):3392-8.

    Insert

    eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    EF1a promoter is upstream of GFP. LoxP in 3'…

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. aCE121

    Plasmid

    #204492

    Purpose

    Cadherin circuit

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    EBFP2
    Cdh6-mKate2
    Cdh1-EYFP
    

    Use

    Synthetic Biology

    Tags

    2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pGBW-m4046310

    Plasmid

    #149022

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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47. pEBB-BT

    Plasmid

    #36295

    Depositor

    Ezra Burstein

    Article

    Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

    Type

    Empty backbone

    Use

    Tags

    BT-tag (peptide sequence from P.freudenreichii tr…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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48. pGBW-m4046662

    Plasmid

    #149221

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. pGBW-m4046228

    Plasmid

    #145400

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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50. pSL1045

    Plasmid

    #129525

    Purpose

    pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    dSpCas9 (Other)
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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