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pGWB553

Plasmid

#74883

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBad-sfGFP 150TAG

Plasmid

#85483

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP 150TAG

Use

Tags

Expression

Bacterial

Mutation

150TAG (amino acid 150 mutated to stop codon)

Promoter

Arabinose

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB433

Plasmid

#74824

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB405

Plasmid

#74799

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pKESaPar

Plasmid

#211829

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB533

Plasmid

#74872

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB444

Plasmid

#74830

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pKERepPar

Plasmid

#211827

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB516

Plasmid

#74858

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-neo

Plasmid

#166467

Purpose

Empty vector to express overexpression cDNA

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK-Zeo

Plasmid

#25736

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pGWB518

Plasmid

#74860

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev

Plasmid

#101340

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat, rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB540

Plasmid

#74874

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB552

Plasmid

#74882

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1.2

Plasmid

#218823

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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7Gli:GFP

Plasmid

#110494

Purpose

reporter of Hedgehog signaling activity

Depositor

Michael Lewis

Article

Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

Insert

7 repeats of the Gli binding site GAACACCCA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

7 repeats of Gli biding site

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB535

Plasmid

#74873

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLC-238

Plasmid

#73192

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-chpB (chpB )

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrhaB

Available since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a)

Plasmid

#79672

Purpose

Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP (Scn9a Mouse, jellyfish)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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