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EGFP-Caveolin-3

Plasmid

#68396

Purpose

Fluorescently tagged Caveolin-3 for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pGEX Grb2 SH2-SH3

Plasmid

#46442

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 (GRB2 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 1-217

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pTOX3

Plasmid

#127448

Purpose

Encodes Tse2 toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

tse2 toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTOX5

Plasmid

#127450

Purpose

Encodes MqsR toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pIC-Cre

Plasmid

#19131

Depositor

Klaus Rajewsky

Article

Gu et al Cell. 1993 Jun 18. 73(6):1155-64.

Insert

Cre recombinase (cre Bacteriophage P1)

Expression

Bacterial

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
AAV-EF1A-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125691

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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SpySwitch

Plasmid

#184225

Purpose

Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpySwitch

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING V155M

Plasmid

#102599

Purpose

Express STING V155M for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

V155M

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDule-pCNF

Plasmid

#85494

Purpose

Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

Insert

para-cyanophenylalanine Mj synthetase

Tags

None

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Noggin

Plasmid

#163586

Purpose

Expresses Noggin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Nog (Nog Mouse)

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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His6-PRC1

Plasmid

#69111

Purpose

For bacterial expression of recombinant human PRC1 tagged with His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Protein Regulator of Cytokinesis 1 (PRC1 Human)

Tags

His6

Expression

Bacterial

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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CTV

Plasmid

#27803

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Traf Venus

Use

Fret standard

Expression

Mammalian

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pAsCpf1(TYCV)(BB) (pY211)

Plasmid

#89352

Purpose

Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CBh

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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EF.delmFasL.CMV.GFP

Plasmid

#17620

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Insert

delFasL (Fasl Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Noncleavable mouse delFasL . Deleted the exon 2 r…

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

Plasmid

#90009

Purpose

Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-DEVD-NanoLuc

Plasmid

#98287

Purpose

caspase-3 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD_Kan-LacI

Plasmid

#79826

Purpose

Arabinose induced expression of Lac repressor

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Insert

LacI

Tags

His6

Expression

Bacterial

Promoter

araBp

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-As-crRNA

Plasmid

#78956

Purpose

Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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S48I48 ELP

Plasmid

#68394

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

S48I48 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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