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pAAV.hSynapsin.mCherry-P2A-Post-SPHERE-SF-iGluSnFR

Plasmid

#187102

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-SF-iGluSnFR

Use

AAV

Tags

mCherry-P2A

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187101

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Use

AAV

Tags

TagBFP-P2A

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPcCas12a

Plasmid

#174681

Purpose

Expresses 6xHis-MBP tagged PcCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPcCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPdCas12a

Plasmid

#174682

Purpose

Expresses 6xHis-MBP tagged PdCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPdCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET23b-proMMP-7-E215Q

Plasmid

#29642

Depositor

William Parks

Article

Ra et al J Biol Chem. 2009 Oct 9. 284(41):27924-3

Insert

proMMP-7 (MMP7 Human)

Expression

Bacterial

Mutation

E215Q Catalytically Inactive

Available Since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
HA_DD-N-mScarlet-I-QtSig

Plasmid

#236775

Purpose

Expresses the red fluorescent protein mScarlet-I fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

HA_DD-N_mScarlet-I_QtSig

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mTagBFP2-QtSig

Plasmid

#236776

Purpose

Expresses the blue fluorescent protein mTagBFP2 fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

DD-N, TagBFP, QtSig

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pE5772

Plasmid

#170779

Purpose

expression of hybrid proteins in E. coli

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisbioMbpTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0F4YNJ0

Plasmid

#163220

Purpose

Inducible expression of UniProt identifier A0A0F4YNJ0

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0F4YNJ0

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huMlCas12a

Plasmid

#174678

Purpose

Expresses 6xHis-MBP tagged MlCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huMlCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huBfCas12a

Plasmid

#174672

Purpose

Expresses 6xHis-MBP tagged BfCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huBfCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSALECT-SupH

Plasmid

#59710

Purpose

pSALECT with SupH insert

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

SupH

Expression

Bacterial

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pSALECT-AhyI

Plasmid

#59708

Purpose

pSALECT with AhyI insert

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

AhyI

Expression

Bacterial

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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3xAP1pGL3 (3xAP-1 in pGL3-basic)

Plasmid

#40342

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

3xAP-1

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains three canonical AP-1 binding sites (TGAC…

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pPLV_C1

Plasmid

#186415

Purpose

Endogenous murine RNase inhibitor production for cell-free lysate

Depositor

Jerome Bonnet

Article

Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

Insert

Murine RNase Inhibitor

Use

Synthetic Biology

Promoter

T7

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Olig001

Plasmid

#170716

Purpose

Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

Depositor

Thomas McCown

Article

Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

Insert

Olig001 capsid gene / rep 2

Use

AAV

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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SPYtag-Histag-Avidin

Plasmid

#214836

Purpose

This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-Histag-Avidin

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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RSVpGL3 (RSV promoter-luciferase in pGL3-basic)

Plasmid

#40343

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

RSV promoter

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

RSV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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SPYtag-GFPnanobody

Plasmid

#214837

Purpose

This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-GFPnanobody

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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eMagB-TgRFP-T

Plasmid

#162252

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-TgRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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