We narrowed to 9,430 results for: try

1. pBad-HPII TAG568

   Plasmid

   #105845

   Purpose

   expresses E. coli HPII catalase with TAG stop codon at site 568 and N-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   hydroperoxidase II catalase
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 568

   Promoter

   pBad/arabinose

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK392

   Plasmid

   #70236

   Purpose

   Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE-H40N

   Depositor

   T. J. Kappock

   Article

   Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   His40Asn

   Promoter

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNJB99

   Plasmid

   #51521

   Purpose

   Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Insert

   Zif268:FokI
   

   Use

   Gateway entry vector

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME pHluorin2

   Plasmid

   #73794

   Purpose

   ratiometric pHluorin2 sequence in a middle entry clone

   Depositor

   David Raible

   Article

   Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

   Insert

   ratiometric pHluorin2
   

   Use

   Middle entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEZT-BM

   Plasmid

   #74099

   Purpose

   Multi-purpose rapid titer bacmam expression vector

   Depositor

   Ryan Hibbs

   Article

   Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Bacmam, baculovirus

   Tags

   EGFP following p10 promoter

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, p10

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. DJ-1 pET15b

   Plasmid

   #60678

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

   Insert

   DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pENTR4-FLAG (w210-2)

   Plasmid

   #17423

   Purpose

   Gateway entry vector for N-term FLAG tag

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pREXC3GH

   Plasmid

   #47077

   Purpose

   FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Lactococcus lactis expression

   Tags

   3C protease site, GFP, and His

   Expression

   Mutation

   Promoter

   NIS (nisin)

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCVpuro-Venus

   Plasmid

   #96940

   Purpose

   Retroviral plasmid containing Venus for labeling of cells

   Depositor

   Lee Grimes

   Article

   Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

   Insert

   Venus Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR4 no ccDB (686-1)

    Plasmid

    #17424

    Purpose

    Gateway entry vector, no ccDB gene

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p-cyclinB1-mcherry (1958)

    Plasmid

    #26063

    Depositor

    Jonathon Pines

    Article

    Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-6xHis

    Plasmid

    #87343

    Purpose

    To express EGFP-6xHis in mammalian cell

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    6xHis
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hs.NRAS G12D

    Plasmid

    #83176

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12D

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1/hChR2-EYFP

    Plasmid

    #20939

    Purpose

    Mammalian expression of humanized ChR2 fused to EYFP

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLew100::NLS-ISceI-HA

    Plasmid

    #21299

    Depositor

    Nina Papavasiliou

    Article

    Boothroyd et al Nature. 2009 May 14;459(7244):278-81. do

    Insert

    I-SceI (SCEI )
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA TfR1-miniR

    Plasmid

    #181986

    Purpose

    MiniR genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniE labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    TfR1-MiniR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-LUC (w158-1)

    Plasmid

    #17473

    Purpose

    Gateway entry vector, Firefly Luciferase

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-CAG-rtTA-IN

    Plasmid

    #60612

    Purpose

    Backbone for tet-inducible genes

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-D-Topo-hTRIM9

    Plasmid

    #51032

    Purpose

    entry vector for cloning human TRIM9

    Depositor

    Timothy Cox

    Article

    Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

    Insert

    TRIM9 (TRIM9 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR4-FKBP12DD N-term A (B3-1)

    Plasmid

    #17414

    Purpose

    Gateway entry vector for Degron, N-term, frame A

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    FKBP12DD (L106P)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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