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p130Cas Y15F

Plasmid

#186154

Purpose

For expressing recombinant p130Cas in insect cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Tags

His6 (Tev)

Expression

Insect

Mutation

15Y residues mutated to Alanine

Promoter

polyhedrin

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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p130Cas

Plasmid

#186131

Purpose

For expression of recombinant p130Cas in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Tags

His6

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-hPGK-mCherry-SynaptoZip

Plasmid

#177317

Purpose

Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

Lentiviral

Tags

Myc and mCherry

Expression

Mammalian

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

Plasmid

#179074

Purpose

Vector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TEF2p-Scarlet(rfp)-Link-2xPH

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Tubb3-GFP KI

Plasmid

#183432

Purpose

CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRB112

Plasmid

#180173

Purpose

pAAV construct GluN1WT (shRNA resistant)

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

AAV

Tags

Phluorin

Expression

Mammalian

Mutation

WT

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-H2B-jGCaMP8s-WPRE

Plasmid

#176749

Purpose

AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

H2B-jGCaMP8s

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-evoA1-seBEN_empty

Plasmid

#174701

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

Lenti-evoA1-seBEN

Use

Lentiviral

Tags

myc tag

Promoter

EFS/U6

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-myc-GBAi

Plasmid

#171753

Purpose

For the mammalian expression of myc-GBAi

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

myc

Expression

Mammalian

Available Since

Nov. 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-evoA1-seBEC

Plasmid

#174702

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

Lenti-evoA1-seBEC

Use

Lentiviral

Tags

flag tag

Mutation

UGI-E11K

Promoter

EFS

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

Plasmid

#171375

Purpose

For the mammalian expression of rat Gαi3 internally tagged with YFP

Depositor

Mikel Garcia-Marcos

Article

Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

Insert

Gai3-YFP

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOTTC1534 pscAAV mU6 shRNA(PKCd) CMV-IE Nuc-EYFP

Plasmid

#135562

Purpose

An AAV vector expressing shRNA vs rat PKCd and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

shRNA (mouse PKCd)
Nuc-EYFP

Use

AAV and RNAi

Tags

3xNLS

Expression

Mammalian

Promoter

CMV-IE and mU6

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DNM2-W525L

Plasmid

#155263

Purpose

Mammalian expression of rat DNM2 fused to HA

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

dynamin 2 (Dnm2 Rat)

Tags

HA

Expression

Mammalian

Mutation

Tryptophan 525 to Leucine

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3-K51A

Plasmid

#155265

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

pEGFP

Expression

Mammalian

Mutation

Lysine 51 to Alanine

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3-R70A

Plasmid

#155264

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

pEGFP

Expression

Mammalian

Mutation

Arginine 70 to Alanine

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat FL Annexin A11

Plasmid

#164496

Purpose

For bacterial expression of rat His-tagged FL Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat FL Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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SnFR-gamma2

Plasmid

#165495

Purpose

Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.

Depositor

Andrew Plested

Article

Hao et al Elife. 2023 Jan 9;12:e84029. doi: 10.755

Insert

iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Synthetic, Rat)

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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E. coli expression: Z-lock cofilin dark state mutant

Plasmid

#141135

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Expression

Bacterial

Mutation

D3 deletion, I32F in Zdk2

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-GLT1a-V5

Plasmid

#162517

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1a (Slc1a2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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BE3-NLS-NLS-P2A-BLAST

Plasmid

#135348

Purpose

Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

BE3-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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