We narrowed to 9,340 results for: UTY

1. SaKKH-ABE8e

   Plasmid

   #138502

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSaCas9 (KKH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMMLV-mRFP1-P2A-Puro

   Plasmid

   #160228

   Purpose

   Expresses mRFP in mammalian cells

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   mRFP1-P2A-Puro
   

   Use

   Retroviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCHC005 (∆RE)

   Plasmid

   #226199

   Purpose

   Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for type I restriction-modification system restriction subunit H16_A0006
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pSD080A

   Plasmid

   #218138

   Purpose

   Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD40-eGFP-IRES2-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGH020_sgRNA_G418-GFP

   Plasmid

   #85405

   Purpose

   hu6 driven sgRNA vector with G418 and GFP selectable markers

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   G418 resistance and GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEYFP-Mitotrap

   Plasmid

   #46942

   Purpose

   Expresses Mitotrap in mammalian cells

   Depositor

   Margaret Robinson

   Article

   Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

   Insert

   Mitotrap (MTOR Human, Aequorea victoria, Synthetic, Budding Yeast)
   

   Use

   Tags

   This is a fusion between a yeast mitochondrial ta…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Human RNF149-3X FLAG

   Plasmid

   #225332

   Purpose

   Lentiviral expression of human RNF149 with 3x FLAG tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   RNF149 (RNF149 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFW2500

   Plasmid

   #224213

   Purpose

   Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Insert

   Fncpf1 and recT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBK1289-AAV-dCjCas9

   Plasmid

   #223138

   Purpose

   AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-SlugCas9-NNG-P2A-puro

    Plasmid

    #214352

    Purpose

    Expresses SlugCas9-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSD103D

    Plasmid

    #218134

    Purpose

    For competition during selection with hybrid circuit. Expresses MBP fused to evolved degron SD55

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD55
    

    Use

    Tags

    MBP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJM189A

    Plasmid

    #218135

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD0
    

    Use

    Tags

    rpoZ

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

    Plasmid

    #135456

    Purpose

    Bacterial expression of uTEV2Δ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability, T30A/S153N mut…

    Promoter

    tac promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

    Plasmid

    #223160

    Purpose

    Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    dGFP-P2A-NanoLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMK444 (EF1-OsTIR1(F74G) Puro)

    Plasmid

    #214374

    Purpose

    EF1-OsTIR1(F74G) (PiggyBac)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    EF1-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV CEPIA4mt

    Plasmid

    #58220

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA4mt
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L F395W D436E

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-eNme2-C.NR

    Plasmid

    #185672

    Purpose

    Expresses nuclease active eNme2-C.NR in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    eNme2-C.NR nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAP2

    Plasmid

    #73211

    Purpose

    express Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)

    Depositor

    Luk Vandenberghe

    Article

    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

    Insert

    AAP2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. esmGFP-C10-pVTCB052

    Plasmid

    #231303

    Purpose

    Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli

    Depositor

    Alexander Rives

    Insert

    esmGFP-C10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-16b-10His-TEV-MCES_VACCA_D1_D12

    Plasmid

    #203671

    Purpose

    The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

    Depositor

    Stephen Cusack

    Article

    Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

    Insert

    MCES_VACCA_D1_D12
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FU-tet-o-hc-myc

    Plasmid

    #19775

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    c-myc (MYC Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28_human-pro-IL18

    Plasmid

    #183390

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. V405 4HA-CKIe

    Plasmid

    #13724

    Depositor

    David Virshup

    Article

    Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

    Insert

    casein kinase 1 epsilon (CSNK1E Human)
    

    Use

    Tags

    4 HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SpCas9-CBE6d-V106W

    Plasmid

    #215827

    Purpose

    Express CBE6d V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6d V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCHC039 (∆phcA)

    Plasmid

    #226207

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for PhcA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. rAAV2/cMG.WPP

    Plasmid

    #184542

    Purpose

    AAV packaging plasmid, expressing Rep2 and AAV-cMG.WPP capsid

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

    Insert

    AAV-cMG.WPP capsid
    

    Use

    AAV; Helper vector for packaging

    Tags

    Expression

    Mutation

    changes on AAV9 capsid: K449R; insertion of WPPKT…

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PAGFP_NS2B3

    Plasmid

    #195755

    Purpose

    Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

    Depositor

    Partha Mondal

    Article

    Varghese et al bioRxiv 2022.12.18.520514

    Insert

    Dengue (Type-2) NS2B3
    

    Use

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    No mutations

    Promoter

    CMV

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSD141B

    Plasmid

    #218139

    Purpose

    Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD56-eGFP-IRES2-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Hy_pMT Flag-URH49 WT

    Plasmid

    #110127

    Purpose

    Expresses Flag-tagged human URH49 (DDX39A)

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    URH49 (DDX39A Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    April 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-pro-IL1b

    Plasmid

    #73953

    Purpose

    Express murine pro-interleukin-1 beta in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

    Insert

    interleukin-1 beta (Il1b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFP-N1-ACTR3

    Plasmid

    #8462

    Depositor

    Matthew Welch

    Article

    Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

    Insert

    ACTR3 (ACTR3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138129

    Purpose

    Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mClover3-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138131

    Purpose

    Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. rAAV2/cMG.QRP

    Plasmid

    #184543

    Purpose

    AAV packaging plasmid, expressing Rep2 and AAV-cMG.QRP capsid

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

    Insert

    AAV-cMG.QRP capsid
    

    Use

    AAV; Helper vector for packaging

    Tags

    Expression

    Mutation

    changes on AAV9 capsid: K449R; insertion of QRPPR…

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5

    Plasmid

    #135455

    Purpose

    Bacterial expression of uTEV1Δ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability, S153N mutation…

    Promoter

    tac promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-MSP1D1deltaH4H5

    Plasmid

    #71716

    Purpose

    Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUb PspCas13b + PspCas13b guide RNA

    Plasmid

    #176305

    Purpose

    Expresses PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E113K, N265S- please depositor comments

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAG201 HIPK1 3'UTR

    Plasmid

    #12012

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    HIPK1 3'UTR (HIPK1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG145 HIPK1 3'UTR

    Plasmid

    #12003

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N10 3'UTR (HIPK1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. FU-tet-o-hKLF4

    Plasmid

    #19777

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    Klf4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pK.myc-MYPT1

    Plasmid

    #24101

    Depositor

    Masumi Eto

    Article

    Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

    Insert

    Myosin-targeting subunit-1 (PPP1R12A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S50G, P415Q

    Promoter

    Available since

    March 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pIRES-Ap2a2-FKBP

    Plasmid

    #46944

    Purpose

    Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Ap2a2-FKBP
    

    Use

    Tags

    Mainly mouse Ap2a2 cDNA, with a fragment of brain…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. mCherry-FKBP-PI4KAC1001(D1957A)

    Plasmid

    #139312

    Purpose

    Recruitable PI4KA kinase-dead catalytic fragment

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D1957A, G1986D

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

    Plasmid

    #190928

    Purpose

    Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Tags

    Strep-TEV-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. Stx4-pmCitrine

    Plasmid

    #92422

    Purpose

    Expression of syntaxin-4 C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGI3EM22C

    Plasmid

    #135488

    Purpose

    Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr LifeAct-tdTomato
    

    Use

    Tags

    LifeAct

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. MBP-TSF-TEVcs-ULK1

    Plasmid

    #171416

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. His_Naa80d in pTYB12

    Plasmid

    #188455

    Purpose

    Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    Naa80 (NAA80 Human)
    

    Use

    Tags

    intein-CBD, 6xHis

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

    Plasmid

    #32520

    Depositor

    Mustafa Sahin

    Article

    Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

    Insert

    Constitutively active Rheb (S16H) (Rheb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S16H to make constitutively active

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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