We narrowed to 9,999 results for: ADA

1. pcS-RDG-C1C2

   Plasmid

   #200163

   Purpose

   This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

   Depositor

   Masako Harada

   Article

   Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

   Inserts

   RDG-C1C2
   C1C2 domain of lactadherin
   

   Use

   Tags

   HA and HIS

   Expression

   Mammalian

   Mutation

   sequence adopted from doi: 10.1371/journal.pone.0…

   Promoter

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEE037

   Plasmid

   #176794

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   406
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKT2/CAGXSP/PalmGamillus

   Plasmid

   #182971

   Purpose

   Stably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   PalmGamillus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MEN2A-MAC

   Plasmid

   #139634

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   MEN2A (RET Human)
   

   Use

   Tags

   StrepIII-HA-BirA*

   Expression

   Mammalian

   Mutation

   C634W

   Promoter

   CMV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GRB2-SHN

   Plasmid

   #139625

   Purpose

   StrepIII-HA-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   GRB2 (GRB2 Human)
   

   Use

   Tags

   StrepIII-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pONL-C1_fibrillarin

   Plasmid

   #65710

   Purpose

   Expresses ONL-fibrillarin in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   fibrillarin (FBL Human)
   

   Use

   Luciferase

   Tags

   ONL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pYNL-C1_fibrillarin

   Plasmid

   #65708

   Purpose

   Expresses YNL-fibrillarin in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   fibrillarin (FBL Human)
   

   Use

   Luciferase

   Tags

   YNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. T7_CC_PE7_IVT_Template

   Plasmid

   #223022

   Purpose

   Template for in vitro transcription of PE7. For HSPC-related experiments.

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PE7

   Plasmid

   #214812

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTTCC

    Plasmid

    #164264

    Purpose

    Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

    Depositor

    Gudmundur Hreggvidsson

    Article

    Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

    Inserts

    Cas9
    gRNA scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pcel and trc

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-PE7 for IVT

    Plasmid

    #214813

    Purpose

    Template for in vitro transcription of PE7

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Promoter

    T7 (inactivated)

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMJ114

    Plasmid

    #85995

    Purpose

    One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified bovine U6-2

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOD2044-intDEG

    Plasmid

    #89366

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Expression

    Mutation

    Promoter

    Pelt-2

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFEB promoter-luciferase reporter

    Plasmid

    #66801

    Purpose

    Reporter for activity of TFEB gene promoter

    Depositor

    Albert La Spada

    Article

    Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

    Insert

    TFEB promoter (Tfeb Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMH0001

    Plasmid

    #85969

    Purpose

    UCOE-SFFV-dCas9-BFP-KRAB

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BFP-KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    SFFV

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-eNme2-T.1-ABE8e

    Plasmid

    #185668

    Purpose

    Expresses eNme2-T.1-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-T.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

    Promoter

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-HA-FbxO5-pcDNA3.1-

    Plasmid

    #52509

    Purpose

    expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO5 (FBXO5 FbxO5, Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-HA-FbxO11-pcDNA3.1-

    Plasmid

    #52501

    Purpose

    expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-eNme2-T.2-ABE8e

    Plasmid

    #185669

    Purpose

    Expresses eNme2-T.2-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-T.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

    Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-EIN-AlkBH5(66-292)

    Plasmid

    #125732

    Purpose

    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

    Depositor

    Vincenzo Venditti

    Article

    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

    Insert

    AlkBH5(66-292) (ALKBH5 Human)
    

    Use

    Tags

    6xHis and EIN

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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