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SIV_Gag_Pol

Plasmid

#236243

Purpose

3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SIV Gag and Pol

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)

Plasmid

#194233

Purpose

Mammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domain

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

CD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-wdb

Plasmid

#195069

Purpose

Expresses FLAG-tagged Drosophila wdb protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

widerborst (wdb Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-S (B.1.351 lineage / South African variant)

Plasmid

#169849

Purpose

Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tag

Depositor

Erik Procko

Article

Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

Insert

Protein S

Use

Tags

HA leader and c-myc tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPN431

Plasmid

#137869

Purpose

Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPN434

Plasmid

#137866

Purpose

Expression of gRNA i2 targeting TCF4 for CRISPRi; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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PEX-LV

Plasmid

#12120

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

noncatalytic fragment of MMP2 comprised of the fi…

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pGateway 3XFlag Prickle2 flag tagged

Plasmid

#24645

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

Prickle 2 (PRICKLE2 Human)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pHAGE GFP-Ago2 S387A

Plasmid

#60654

Purpose

GFP-Ago2 S387A mutant lentiviral vector

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

S387A

Promoter

EF1a

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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Lrrc4-Fc-His

Plasmid

#72084

Purpose

Expresses the extracellular region of the LRRC4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Lrrc4 (Lrrc4 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-W-FUGW

Plasmid

#166751

Purpose

Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Bcl-W (Bcl2l2 Mouse)

Use

Lentiviral

Tags

mCerulean3

Expression

Mutation

Promoter

human ubiquitin C

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE GFP-Ago2 S387E

Plasmid

#60655

Purpose

GFP-Ago2 S387E mutant lentiviral vector

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

S387E

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Jam2-AP-His

Plasmid

#71953

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Lrrc4-AP-His

Plasmid

#71958

Purpose

Expresses the extracellular region of the LRRC4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Lrrc4 (Lrrc4 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry Tensin2

Plasmid

#105300

Purpose

To study localization and function of Tensin 2 with EGFP Tensin1

Depositor

Kenneth Yamada

Article

Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

Insert

Tensin2 (TNS2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

lacks N-terminal C1 domain

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2

Plasmid

#79255

Purpose

Expresses EGFP-tagged DmNot2 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot2 (Rga Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCCr4

Plasmid

#79252

Purpose

Expresses EGFP-tagged DmCCr4 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmCaf40 (twin Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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Jam2-Fc-His

Plasmid

#72079

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-PLOD2_PGK-GpNLuc

Plasmid

#136457

Purpose

Expresses Tet/Dox-inducible human PLOD2 in mammalian cells.

Depositor

Antonio Amelio

Article

Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

Insert

TRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)

Use

Luciferase and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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