We narrowed to 4,921 results for: puc

1. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42229

   Purpose

   This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR-eGFP-attP(bxb)-*BsdR

   Plasmid

   #183751

   Purpose

   Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBR_attB(bxb)_ccdB_lox

   Plasmid

   #183763

   Purpose

   Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   ccdB
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Bxb1_AJMA

   Plasmid

   #183760

   Purpose

   Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Inserts

   ASAP2f
   jRCaMP1b
   miRFP703
   ASAP2f
   

   Use

   Tags

   Lck and NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42337

   Purpose

   Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

   Plasmid

   #99692

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Hbb (Hbb Synthetic)
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KG#203

   Plasmid

   #110880

   Purpose

   Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally

   Depositor

   Kenneth Miller

   Article

   Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

   Inserts

   rab-3 promoter
   pde-4d (isoform d) cDNA
   unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR-mCherry-attP(C31)-*BleoR

   Plasmid

   #183752

   Purpose

   phiC31 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   FRT-mCherry-attP-BleoR-F3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYL236

   Plasmid

   #173184

   Purpose

   CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 (EZH2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAcCas12nHS_RV6_PT1_empty

    Plasmid

    #203810

    Purpose

    Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12nHS_RV6_PT1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-flex-rev-g2-2A-Venus

    Plasmid

    #71410

    Purpose

    This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.

    Depositor

    William Wisden

    Article

    Uygun et al J Neurosci. 2016 Nov 2;36(44):11171-1118

    Inserts

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse hdc gene promoter, works as a pan neuronal …

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV-SynCas.v47 mammalian expression

    Plasmid

    #209003

    Purpose

    Expression of SynCas.v47 for analysis of RNA knockdown

    Depositor

    Omar Akbari

    Article

    Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

    Insert

    SynCas.v47
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMV-SynCas.v10 mammalian expression

    Plasmid

    #208589

    Purpose

    Expression of SynCas.v10 for analysis of RNA knockdown

    Depositor

    Omar Akbari

    Article

    Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

    Insert

    SynCas.v10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CMV-SynCas.v14A mammalian expression

    Plasmid

    #208590

    Purpose

    Expression of SynCas.v14A for analysis of RNA knockdown

    Depositor

    Omar Akbari

    Article

    Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

    Insert

    SynCas.v14A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-SynCas.v35 mammalian expression

    Plasmid

    #208591

    Purpose

    Expression of SynCas.v35 for analysis of RNA knockdown

    Depositor

    Omar Akbari

    Article

    Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

    Insert

    SynCas.v35
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-SynCas.v39 mammalian expression

    Plasmid

    #208592

    Purpose

    Expression of SynCas.v39 for analysis of RNA knockdown

    Depositor

    Omar Akbari

    Article

    Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

    Insert

    SynCas.v39
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-SynCas.v41 mammalian expression

    Plasmid

    #208593

    Purpose

    Expression of SynCas.v41 for analysis of RNA knockdown

    Depositor

    Omar Akbari

    Article

    Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

    Insert

    SynCas.v41
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRA1SEGFPTcIfm

    Plasmid

    #193777

    Purpose

    Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PR promoter and PLTetO-1

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS1930

    Plasmid

    #79779

    Purpose

    High copy number pUC vector containing Ple257 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple257
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple257 (PAX6 MiniPromoter)

    Available since

    Nov. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAd388-BPTF-sh28

    Plasmid

    #83276

    Purpose

    shRNA targeting BPTF

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA to BPTF
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    pUC ori

    Available since

    Nov. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge