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Plasmid#123355PurposeYellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.DepositorInsertVenus-cpVenus-FLARE-ICUE
UseTags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Flex-TREx2-tTA
Plasmid#191210PurposeCre dependent TRE-tTA expression under the control of Syn promoterDepositorInserttTA
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPHK2 gRNA (BRDN0001148465)
Plasmid#77093Purpose3rd generation lentiviral gRNA plasmid targeting human SPHK2DepositorInsertSPHK2 (SPHK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MbPylRS(DiZPK)
Plasmid#91706Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interestDepositorInsertspylS
pylT
UseTagsExpressionMammalianMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257APromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
HBx
Plasmid#65463Purposeexpresses HBx in mammalian cellsDepositorInsertHBV X protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGST2-Rabex5 (9-73)
Plasmid#17630DepositorInsertRabex5 (9-73) (RABGEF1 )
UseTagsGSTExpressionBacterialMutationUbiquitin-binding domain: residues 9-73PromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_T
Plasmid#74068PurposepAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecRDepositorInsertsgRNA pck (T)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYC-C
Plasmid#207114PurposeHDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locusDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
Plasmid#203229PurposeCre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertCre-dependent, membrane-localized Optopatch
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PECAM1-COMP5AP-AviTag-9xHis
Plasmid#157481PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPECAM1 (PECAM1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only