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Plasmid#164985PurposeNPRL2 expressing vector for mammalian cellsDepositorInsertNPRL2 (NPRL2 Human)
UseTags2xSTREP FLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TWIN-STREP-FLAG-NPRL3
Plasmid#164984PurposeNPRL3 expressing vector for mammalian cellsDepositorInsertNPRL3 (NPRL3 Human)
UseTags2xSTREP FLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 1x perfect to seed plus 13-16 siRNA
Plasmid#40767PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsertCXCR4 small RNA perfect to seed plus 13-16 target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK-hFluc-CrPV-hRluc-6xbsMS2-Malat_AC
Plasmid#148443PurposeMammalian Expression of 6xbsMS2-MalatDepositorInsert6xbsMS2-Malat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK-hFluc-HCV-hRluc-6xbsMS2-Malat_AC
Plasmid#148444PurposeMammalian Expression of 6xbsMS2-MalatDepositorInsert6xbsMS2-Malat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized
Plasmid#184563PurposeCMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.DepositorInsertC16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
UseLentiviralTagsExpressionMammalianMutationAll lysines were mutated to arginines. Codon-opti…PromoterCMVAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)
Plasmid#166421PurposeExpress pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)DepositorInsertNaegleria fowleri PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)
Plasmid#166422PurposeExpress pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)DepositorInsertPlasmodium falciparum PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)
Plasmid#166423PurposeExpress pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)DepositorInsertPythium oligandrum PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)
Plasmid#166424PurposeExpress pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)DepositorInsertReticulomyxa filosa PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)
Plasmid#166425PurposeExpress pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)DepositorInsertSphaeforma arctica PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)
Plasmid#166415PurposeExpress pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)DepositorInsertAcytostelium subglobosum PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)
Plasmid#166416PurposeExpress pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)DepositorInsertcaenorhabditis elegans PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Danio rerio PRL (Δprenylation motif)
Plasmid#166417PurposeExpress pET-15b(+) Danio rerio PRL (Δprenylation motif)DepositorInsertDanio rerio PRL (ptp4a3a Zebrafish)
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)
Plasmid#166418PurposeExpress pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)DepositorInsertDrosophila melanogaster PRL (Prl Fly)
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)
Plasmid#166419PurposeExpress pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)DepositorInsertGaldieria sulphuraria PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)
Plasmid#166420PurposeExpress pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)DepositorInsertMonosiga brevicollis PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Oprl1
Plasmid#184291PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Oprl1DepositorInsertsgRNA-Oprl1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Mortierella verticillate (delta prenylation motif)
Plasmid#170788PurposeExpresses pET-15b(+) Mortierella verticillate (delta prenylation motif)DepositorInsertMortierella verticillate PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Toxoplasma gondii (delta prenylation motif)
Plasmid#170789PurposeExpresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)DepositorInsertToxoplasma gondii PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only