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Plasmid#101668PurposeOpto-T7RNAP*(563-F2), half expr.DepositorInsertOpto-T7RNAP*(563-F2), half expr.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB171
Plasmid#101670PurposeOpto-T7RNAP*(69), equal expr.DepositorInsertOpto-T7RNAP*(69), equal expr.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJin-290
Plasmid#125718PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-CDepositorInsertPCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS6
Plasmid#55752PurposeProduces T7 RNA Polymerase.DepositorInsertT7 RNA Polymerase
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5-79
Plasmid#118087PurposeN-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmidDepositorInsertN-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5-71
Plasmid#118085PurposeN-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmidDepositorInsertN-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pTHSSd_27
Plasmid#59958PurposePTac null fragment (sigma CGG Y639A) expressionDepositorInsertnull fragment
UseTagsExpressionBacterialMutationN/APromoterPTacAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_38
Plasmid#59961PurposeHigh core fragment expression (high resource allocator)DepositorInsertcore fragment
UseTagsExpressionBacterialMutationN/APromoterPJ23105Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_1
Plasmid#59947PurposeSplitposon in KanR ColE1 backboneDepositorInsertSplitposon w/ KanR, LacI & PTac
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_39
Plasmid#59962PurposeLow core fragment expression (low resource allocator)DepositorInsertcore fragment
UseTagsExpressionBacterialMutationN/APromoterPJ23105Available sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_40
Plasmid#59963PurposeHigh core fragment expression without SynZIPDepositorInsertcore fragment lacking SynZIP
UseTagsExpressionBacterialMutationN/APromoterPJ23105Available sinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
PIA586
Plasmid#104399Purposevector for expression of single E.coli RNAP subunitDepositorInsertrpoD
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
UseTags14Histidine-TEVExpressionBacterialMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Neq2X7
Plasmid#182366PurposeExpresses Neq2X7 DNA polymeraseDepositorInsertNeq2X7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT2dR3-proB
Plasmid#122595PurposeBE PACE selection, express T7 RNA polymerase with degron-GTCCA targetDepositorInsertT7 RNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pARiBo4
Plasmid#98893PurposeExpresses ARiBo-fused RNA in vitro with T7 RNA polymeraseDepositorInsertARiBo4
UseIn vitro transcription vectorTagsExpressionMutationPromoterT7 promoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEW13
Plasmid#24007DepositorInsertT7 RNA Polymerase
UseTrypanosome expressionTagsExpressionMutationPromoterAvailable sinceMay 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pETMCSI-T4P_Y320A
Plasmid#122522PurposeExpression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesisDepositorInsertgp43 - DNA polymerase (43 Escherichia virus T4)
UseTagsExpressionBacterialMutationtyrosine 320 changed to alaninePromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only