Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 241 - 280 of 392 results

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pW5Y_Apr

Plasmid

#158211

Purpose

BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites

Depositor

Yasuo Yoshikuni

Article

Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

Type

Empty backbone

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Strep-FLAG KBP

Plasmid

#236459

Purpose

transient overexpression of KBP in mammalian cells

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

KBP (KIF1BP Human)

Tags

Strep-FLAG

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-GFP

Plasmid

#181906

Purpose

Fluorescently tagged dead Cas9

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-HSPA5P

Plasmid

#44150

Purpose

a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

Depositor

Joan Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

HSPA5 promoter (HSPA5 Human)

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-CAT

Plasmid

#173943

Purpose

Bacterial expression of isolated PARP1 CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-Voltron

Plasmid

#119035

Purpose

Cre-dependant expression of Voltron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

AAV

Expression

Mammalian

Promoter

hsyn

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-FLJ10081

Plasmid

#64775

Purpose

Mammalian expression of Flag-tagged FLJ10081 (NSL3)

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

FLJ10081 (KANSL3 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-KIAA0947

Plasmid

#49248

Purpose

Mammalian expression of flag-tagged human KIAA0947

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

KIAA0947 (ICE1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delBRCT

Plasmid

#173938

Purpose

Bacterial expression of PARP1 lacking BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delBRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 374-520; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn2

Plasmid

#174805

Purpose

Bacterial expression of isolated PARP1 Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn2 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn3

Plasmid

#174806

Purpose

Bacterial expression of isolated PARP1 Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn3 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xSTREP Cyclin F(LP35/36AA)

Plasmid

#236467

Purpose

transient overexpression of Cyclin F in mammalian cells

Depositor

Michele Pagano

Article

D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

Insert

Cyclin F (CCNF Human)

Tags

2xSTREP

Expression

Mammalian

Mutation

(LP35/36AA) first two amino acids of the F-box do…

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn2

Plasmid

#173935

Purpose

Bacterial expression of PARP1 lacking Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn2 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 97-213 (V762A)

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-Flag-Cul5

Plasmid

#31984

Purpose

Mammalian expression of Flag-tagged Cul5

Depositor

Joan Conaway

Article

Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

Insert

Cul5 (CUL5 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delCAT

Plasmid

#173942

Purpose

Bacterial expression of PARP1 lacking CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delCAT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 661-1014

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delWGR

Plasmid

#173940

Purpose

Bacterial expression of PARP1 lacking WGR domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delWGR (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 521-660; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-Flag-NRBP1

Plasmid

#48197

Purpose

Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1)

Depositor

Joan Conaway, Ronald Conaway

Article

Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

Insert

NRBP1 (NRBP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delZn3

Plasmid

#173936

Purpose

Bacterial expression of PARP1 lacking Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn1

Plasmid

#173933

Purpose

Bacterial expression of PARP1 lacking Zn1 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-HD

Plasmid

#174809

Purpose

Bacterial expression of isolated PARP1 HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-HD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-hINO80N2 (1-406)

Plasmid

#29442

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1-406 (a.a.)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/Flag-hINO80N1 (1-262)

Plasmid

#29441

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1-262 (a.a.)

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT to BioID-nDIC2-3xFlag

Plasmid

#232418

Purpose

Expresses human dynein subunit DIC2 in mammalian cells.

Depositor

Samara Reck-Peterson

Article

Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

Insert

Dynein Intermediate Chain 2 (DYNC1I2 Human)

Tags

BioID and Flag-3x

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT to BioID-cDIC2-3xFlag

Plasmid

#232416

Purpose

Expresses human dynein subunit DIC2 in mammalian cells.

Depositor

Samara Reck-Peterson

Article

Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

Insert

Dynein Intermediate Chain 2 (DYNC1I2 Human)

Tags

BioID and Flag-3x

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-GAL4-PARP1-CAT-L713F

Plasmid

#175949

Purpose

Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

GAL4 DNA binding domain (GAL4 Budding Yeast)

Tags

6xHis tag

Expression

Bacterial

Mutation

L713F, V762A for the 'PARP1-CAT-L713F' …

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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CEA-N domain residues 1-132 (CEACAM5)-pET30b

Plasmid

#174693

Purpose

Plasmid expressing CEA-N domain

Depositor

Jean Gariepy

Article

Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

Insert

CEA-N Domain

Tags

TEV Cleavage Site and hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1deltaART

Plasmid

#174804

Purpose

Bacterial expression of an inactive PARP1 mutant lacking the ART subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1deltaART (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 785-1014

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pW17

Plasmid

#158207

Purpose

2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

Depositor

Yasuo Yoshikuni

Article

Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

Insert

loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Med25

Plasmid

#64771

Purpose

Mammalian protein expression of flag-tagged Med25

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25 (MED25 Human)

Tags

Flag

Expression

Mammalian

Promoter

pCMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-hsyn-flex-Voltron-ST

Plasmid

#119036

Purpose

Cre-dependant expression of soma-localized Voltron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Has Service

AAV1

Insert

Voltron-ST

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hsyn

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-F-Med26-NTD

Plasmid

#49255

Purpose

Mammalian expression of flag-tagged Med26-NTD

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

NTD (aa 1-339)

Available Since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5-F-Med26-R61A K62A

Plasmid

#49254

Purpose

Mammalian expression of flag-tagged human Med26 R61A K62A

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

R61A K62A

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5-Flag-Med26

Plasmid

#49250

Purpose

Mammalian expression of flag-tagged Med26

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1delZn2delBRCT

Plasmid

#173939

Purpose

Bacterial expression of PARP1 lacking Zn2 and BRCT domains

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn2delBRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 97-213 and 374-520; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-CAT-L713F

Plasmid

#173944

Purpose

Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-Med25-VBD-6His

Plasmid

#64773

Purpose

Bacterial expression of His-tagged Med25-VBD

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25-VBD (MED25 Human)

Expression

Bacterial

Mutation

insert is 394-543 only

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-L713F

Plasmid

#174794

Purpose

Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 241 - 280 of 392 results

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