-
Plasmid#64611Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertSTAT3 (STAT3 Human)
UseLentiviralTagsV5ExpressionMutationA662C, N664C, V667LPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gli2 truncation-pcw107-V5
Plasmid#64626Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertGLI2 (truncation mutant) (GLI2 Human)
UseLentiviralTagsV5ExpressionMutationtruncatedPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Caspase-3 (C163A)-pcw107-V5
Plasmid#64633Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCASP3 (transcript variant beta) (CASP3 Human)
UseLentiviralTagsV5ExpressionMutationC163APromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
JNK2 WT O/E (MAPK9)-pcw107-V5
Plasmid#64617Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertMAPK9 (transcript variant JNK2-a2) (MAPK9 Human)
UseLentiviralTagsV5ExpressionMutationnonePromoterPGKAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
SmoM2 (W535L)-pcw107
Plasmid#64627Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertSMO (SMO Human)
UseLentiviralTagsExpressionMutationW535LPromoterPGKAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
myr-FLAG-MEK5-pcw107
Plasmid#64620Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertMAP2K5 (MAP2K5 Human)
UseLentiviralTagsFlag and myristoylatedExpressionMutationPromoterPGKAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKK6 (S207E,T211E)-pcw107
Plasmid#64625Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertMAP2K6 (MAP2K6 Human)
UseLentiviralTagsFLAGExpressionMutationS207E,T211EPromoterPGKAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-YAP2 (8SA)-pcw107
Plasmid#64637Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertYAP2 (transcript variant 3) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMutation8SA*PromoterPGKAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant
Plasmid#72924PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationSerine#37 changed to Alanine;Silent mutations mad…PromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant
Plasmid#72617PurposeExpresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseLentiviralTags3XAU1 and 3XFLAGExpressionMammalianMutationSerine#37 changed to Alanine;Silent mutations mad…PromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
HRas (G12V, E37G)-pcw107
Plasmid#64639Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertHRAS (transcript variant 3) (HRAS Human)
UseLentiviralTagsExpressionMutationG12V, E37GPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HRas (G12V, E37G)-pcw107-V5
Plasmid#64640Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertHRAS (transcript variant 3) (HRAS Human)
UseLentiviralTagsV5ExpressionMutationG12V, E37GPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR A757
Plasmid#140829PurposeAIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757DepositorInsertAIMTOR A757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
SV.signed
Plasmid#133933PurposeTo validate the use of digital signatures in DNA.DepositorInsertlacZ and digital signature cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
CS2 Flag-Smad6
Plasmid#14961DepositorInsertSmad6 (SMAD6 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationPromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
CS2 HA-Smad6
Plasmid#14962DepositorInsertSmad6 (SMAD6 Human)
UseXenopus/mammalian/avian/zebrafishTagsHAExpressionMutationS18LPromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
-
p1990 SV40-LMP1
Plasmid#26654DepositorInsertLatent Membrane Protein 1 (LMP-1 Epstein-Barr Virus)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSP64T BMPR (DM#136)
Plasmid#15068DepositorInsertBMP receptor (bmpr1a Frog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMV2 BMPR (DM#138)
Plasmid#15069DepositorInsertBMP receptor (bmpr1a Frog)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
KG#62
Plasmid#110874PurposeExpresses the C. elegans acy-1 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Caspase-1 (C285A)
Plasmid#131353PurposeExpresses Caspase-1 C285ADepositorInsertCaspase-1 (C285A) (Casp1 Mouse)
UseRetroviralTagsExpressionMammalianMutationCaspase-1 (C285A)PromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 hSmad1-EVE
Plasmid#22993DepositorInsertSmad1 (SMAD1 Human)
UseTagsFLAGExpressionMammalianMutationS463E, S465EPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCS2 hSmad1-EVE-MM
Plasmid#22994DepositorInsertSmad1 (SMAD1 Human)
UseTagsFLAGExpressionMammalianMutationS214A, S206A, S195A, S187A, S463E, S465EPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf (XSB522)
Plasmid#78512PurposeConstruct for expressing His10 - SNAPf in E. ColiDepositorInsertSNAPf
UseTagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutationPromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2 hSmad1-GM
Plasmid#22992DepositorInsertSmad1 (SMAD1 Human)
UseTagsFLAGExpressionMammalianMutationS210A ,T202A, S191A, S198APromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-CREB
Plasmid#15221DepositorInsertCREB (Creb1 Rat)
UseTagsGal4ExpressionMammalianMutationPromoterAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pH6-spTorA-mCherry
Plasmid#169040PurposemCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.DepositorInsertH6-spTorA-mCherry
UseTags6xHis and spTorA (signal peptide of E. coli TorA)ExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KG#45
Plasmid#110926PurposeVector for expressing proteins in most or all tissues, including nervous system, under control of the inducible heat shock promoter in C. elegansDepositorInsertshsp-16-2 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSP64T tBMPR (DM#135)
Plasmid#15067DepositorInsertBMP receptor (bmpr1a Frog)
UseTagsExpressionBacterialMutationTruncated dominant negative formPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-MyD88-Rip3
Plasmid#131352PurposeExpresses MyD88 with Rip3 fused to its C-terminusDepositorInsertMyD88-Rip3 (Myd88 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKalpha FL WT
Plasmid#33327DepositorInsertCalcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
UseTagsFLAGExpressionMammalianMutationPromoterSV40Available sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf-9E (XSB526)
Plasmid#78514PurposeConstruct for expressing His10 - SNAPf -9E in E. ColiDepositorInsertSNAPf
UseTagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutation9E added to the C-terminal of SNAPfPromoterAvailable sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
-
KG#80
Plasmid#110875PurposeExpresses the C. elegans acy-1 cDNA in body wall muscleDepositorInsertsmyo-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf-9R (XSB525)
Plasmid#78513PurposeConstruct for expressing His10 - SNAPf -9R in E. ColiDepositorInsertSNAPf
UseTagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutation9R added to the C-terminal of SNAPfPromoterAvailable sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS BMPR (DM#137)
Plasmid#15059DepositorInsertBMP receptor (bmpr1a Frog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLp_3050sNuc
Plasmid#122030PurposePlasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakeiDepositorInsertsignal peptide Lp_3050
UseTagsExpressionBacterialMutationPromotersppAAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-delta30-TFEB
Plasmid#44445DepositorInsertTFEB (TFEB Human)
UseTagsGFPExpressionMammalianMutationDeleted amino acids 1-30; H32DPromoterAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pegfp-N1-TFEB-S3A,R4A
Plasmid#44446DepositorInsertTFEB (TFEB Human)
UseTagsGFPExpressionMammalianMutationChanged both Serine 3 and Arginine 4 to AlaninePromoterCMVAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-TRAF6-targeting sgRNA 1
Plasmid#131345PurposegRNA targeting mouse TRAF6DepositorInsertgRNA targeting mouse TRAF6 (Traf6 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-CREB S133A
Plasmid#15222DepositorInsertCREB S133A (Creb1 Rat)
UseTagsGal4ExpressionMammalianMutationSerine 133 mutated to AlaninePromoterAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-TRAF6-targeting sgRNA 2
Plasmid#131346PurposegRNA targeting mouse TRAF6DepositorInsertgRNA targeting mouse TRAF6 (Traf6 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-3xFLAG-MyD88-V5
Plasmid#131351PurposeExpresses MyD88 tagged with a 3xFLAG tag at its N-terminus and a V5 tag at its C-terminusDepositorInsertMyD88 (Myd88 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
CS2 Flag-OAZ
Plasmid#14939DepositorInsertOAZ (ZNF423 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationPromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-MyD88-NpLxIS
Plasmid#131347PurposeExpresses MyD88 with 2 copies of pLxIS motif from STING fused to its N-ternimusDepositorInsertMyD88-NpLxIS (Myd88 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only