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Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179117

Purpose

Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Fermt2 sgRNA

Use

AAV

Tags

Expression

Mutation

Promoter

U6

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

Plasmid

#178960

Purpose

Expresses saCas9 protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Staphylococcus aureus Cas9 (SaCas9)

Use

AAV

Tags

3x HA

Expression

Mutation

Promoter

gfaABC1D (astrocyte specific)

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-AC3CtermGenomic-mCherry

Plasmid

#105250

Purpose

mouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

mCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACt-mCherry-NLS

Plasmid

#138214

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NLS (Adcy10 Rat)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACt-mCherry-NES

Plasmid

#138215

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NES (Adcy10 Rat)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-sACt-mCherry

Plasmid

#138216

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

Lyn-sACt-mCherry (Adcy10 Rat)

Use

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC13A2

Plasmid

#131921

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC13A2 (SLC13A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC13A2_STOP

Plasmid

#161087

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC13A2 (SLC13A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pORFE0003

Plasmid

#112072

Purpose

AtDeadCAS9 D10A H840A module CDS1

Depositor

Christian Chevalier

Insert

AtDeadCAS9 D10A H840A

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized dCas9 D10A S. pyogenes

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pORFE0005

Plasmid

#112073

Purpose

AtDeadCAS9 D10A H840A without stop codon module CDS1ns

Depositor

Christian Chevalier

Insert

AtDeadCAS9 D10A H840A without stop codon

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized dCas9 D10A S. pyogenes

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBL069B

Plasmid

#194802

Purpose

cCadC-GCN4* under the control of Ptet

Depositor

Tina Wang

Article

Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

Insert

cCadC-GCN4*

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBL090J

Plasmid

#195045

Purpose

cCadC-CCB3.5 under the control of PBAD

Depositor

Tina Wang

Article

Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

Insert

cCadC-CCB3.5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBL069

Plasmid

#194800

Purpose

cCadC-GCN4 under the control of Ptet

Depositor

Tina Wang

Article

Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

Insert

cCadC-GCN4

Use

Luciferase and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSARSF53-343

Plasmid

#61759

Purpose

TvoVMAdC48 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAdC48 intein

Use

Tags

H6-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-143

Plasmid

#61760

Purpose

TvoVMAdC16 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAdC16 intein

Use

Tags

H6-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-HY176

Plasmid

#61761

Purpose

TvoVMAdC7 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAdC7 intein

Use

Tags

H6-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SF-1 shRNA #1

Plasmid

#28186

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
SF-1 shRNA #2

Plasmid

#28187

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
SF-1 shRNA #3

Plasmid

#28188

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pJK_kanR_proJK1_PAD

Plasmid

#60630

Purpose

Constitutively expresses PAD (phenolic acid decarboxylase)

Depositor

Timothy Whitehead

Article

Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

Insert

Phenolic acid decarboxylase padC (padC )

Use

Tags

Expression

Bacterial

Mutation

K146Y

Promoter

proJK1

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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