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pXPR_003 sgMYC guide 2

Plasmid

#193597

Purpose

cMYC knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgMYC guide 2 (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MDH1-shDusp5-2-H2Kb2

Plasmid

#17853

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

shDusp5 (DUSP5 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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2. p167 Dync1i1.D

Plasmid

#26439

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform D (Dync1i1 Mouse)

Use

Subcloning and sequencing

Tags

Expression

Mutation

N/A

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pXPR_003 sgGPAA1 guide 2

Plasmid

#193609

Purpose

GPAA1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgGPAA1 guide 2 (GPAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgAP2M1 guide 2

Plasmid

#193601

Purpose

AP2M1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgAP2M1 guide 2 (AP2M1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgFADD guide 2

Plasmid

#193590

Purpose

FADD knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgFADD guide 2 (FADD Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgRELA guide 2

Plasmid

#193592

Purpose

RELA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRELA guide 2 (RELA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcdna DSG-2 TS

Plasmid

#89465

Purpose

DSG-2 tension sensor, using TSmod inserted in human DSG-2

Depositor

Daniel Conway

Article

Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

Insert

desmoglein-2 (DSG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

Plasmid

#107727

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-SwitchON-sgCh2-2

Plasmid

#199637

Purpose

Tamoxifen-inducible expression of sgRNA control targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgINTS12 guide 2

Plasmid

#193607

Purpose

INTS12 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgINTS12 guide 2 (INTS12 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX330A-Yap1-E1-#2

Plasmid

#171519

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSicoR (EGFP) shPnky-2

Plasmid

#79142

Purpose

Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre

Depositor

Daniel Lim

Article

Ramos et al Cell Stem Cell. 2015 Apr 2;16(4):439-47.

Insert

shPnky-2 (Gm30731 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 for the shRNA and CMV for EGFP

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2

Plasmid

#164435

Purpose

Encodes SARS-CoV-2 Spike Protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pHDM-SARS-CoV-2

Plasmid

#164433

Purpose

Encodes SARS-CoV-2 Spike Protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

Plasmid

#107730

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shHRASLS.2

Plasmid

#110324

Purpose

TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

HRASLS (PLAAT1 Homo sapiens, Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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PL-CRISPR.EFS.tRFP-gIfngr1-2

Plasmid

#166480

Purpose

Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Ifngr1 (Ifngr1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Spike Display_Part 2 Spacer

Plasmid

#172730

Purpose

Encodes Part 2 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Tags

Expression

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Promoter

Available since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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