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Plasmid#70742Purpose1 color weak reporter used in studyDepositorInsertsfGFP-ssrA
UseTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102892PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)
Plasmid#50032PurposeGalactose-inducible expression of ER-luminal S. pyogenes SrtA in yeastDepositorInsertHA-SrtA(strep)
UseTagsHA-HDELExpressionYeastMutationPromoterpGALAvailable sinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-BCD20-Citrine-ssrA P64U23C88T18 v1-1 (586)
Plasmid#70743Purposefor building 3-color reporter plasmidDepositorInsertCitrine-ssrA
UseTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-RGG-GFP-RGG-mHP1alpha
Plasmid#181893PurposeSoluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mustard Weed, Mouse)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-EF1α-Luc-C20orf24-3’UTR-VAR
Plasmid#128507PurposeLentiviral constitutive expression of Firefly luciferase under control of 3'UTR of human C20orf24 with variant from COX deficiency patient.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry
Plasmid#203652PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.DepositorArticleInsertPfV
UseLentiviralTagsSapphire-IRES-H2B-mCherryExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.Flag.p34
Plasmid#39221DepositorInsertp34 (GTF2H3 Human)
UseTagsFlagExpressionInsectMutationPromoterPphAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCoofy27
Plasmid#44003DepositorTypeEmpty backboneUseTagsHis6ExpressionInsectMutationPromoterPPHAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.His.XPD
Plasmid#39217DepositorInsertXPD (ERCC2 Human)
UseTagsHis (HHYHHH)ExpressionInsectMutationXPD starts at aa4PromoterPphAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.His.XPB
Plasmid#39216DepositorInsertXPB (ERCC3 Human)
UseTagsHisExpressionInsectMutationPromoterPphAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCoofy28
Plasmid#44004DepositorTypeEmpty backboneUseTagsHis6-GSTExpressionInsectMutationPromoterPPHAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy29
Plasmid#44005DepositorTypeEmpty backboneUseTagsHis6-MBPExpressionInsectMutationPromoterPPHAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy41
Plasmid#55184PurposeInsect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTagsHis10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…ExpressionBacterialMutationPromoterpPH (Polyhedrin promoter)Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_ENO1-Halo
Plasmid#175330PurposeProduction of HaloTag fusion proteinDepositorInsertENO1 (ENO1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCoofy44
Plasmid#55185PurposeInsect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…ExpressionBacterialMutationPromoterpPH (Polyhedrin promoter)Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2.1 (pAGL2A)
Plasmid#27563DepositorInsertENO1 promoter (ENO1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-ENO1_sgRNA1
Plasmid#201592PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertENO1 (ENO1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-ENO1_sgRNA2
Plasmid#201593PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertENO1 (ENO1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
IBMc460
Plasmid#161940PurposeCarries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoterDepositorInsertpSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
UseTagsExpressionBacterialMutationPromoterPPhlFAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only