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pBNJ28.2

Plasmid

#66874

Purpose

For transposon tagging with YFP

Depositor

Thomas Hughes

Article

Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

Insert

eYFP in a transposon

Use

Source for a tn7 transposon

Tags

eYFP

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBNJ26.3

Plasmid

#66876

Purpose

For CFP tagging via transposon insertion.

Depositor

Thomas Hughes

Article

Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

Insert

eCFP in a transposon

Use

Source for a tn7 transposon

Tags

eCFP

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pVB005

Plasmid

#127189

Purpose

Tuner sRNA booster

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

sRNA Booster

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRH8

Plasmid

#101014

Purpose

dominant negative glutamate receptor subunit

Depositor

Thomas Hughes

Article

Robert et al Neuroscience. 2002;115(4):1199-210.

Insert

glutamate

Expression

Mammalian

Mutation

CHANGED THE GLUR USING PRIMERS FOUND IN ROBERT ET…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-PAmCherry

Plasmid

#133412

Purpose

Fluorescent reporter for calcium imaging and photoactivation.

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-PAmCherry

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP5g-P2A-PAmCherry

Plasmid

#133411

Purpose

Fluorescent reporter for calcium imaging and photoactivation.

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pM5HHB5

Plasmid

#17674

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src (SRC Chicken)

Expression

Mammalian

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pVB006

Plasmid

#140327

Purpose

Tunable NOR gate

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

Tunable NOT gate and Reporter

Expression

Bacterial

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSTC1

Plasmid

#39241

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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PpPchE

Plasmid

#242092

Purpose

Nonribosomal peptide synthetase from Pseudomonas protegens for the production of enantiopyochelin

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

PpPchE

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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SvPchE

Plasmid

#242093

Purpose

Nonribosomal peptide synthetase from Streptomyces venezuluelae for the production of watasemysin

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

SvPchE

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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BsSFP

Plasmid

#242094

Purpose

4'phosphopantetheinyl transferase from Bacillus subtilis

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

BsSFP

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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BsSrfD

Plasmid

#242095

Purpose

Thioesterase from Bacillus subtilis

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

BsSrfD

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVB002

Plasmid

#127186

Purpose

Tunable NOT gate with RiboJ insulator

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

Tunable NOT gate with RiboJ and Reporter

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pREMORAv1

Plasmid

#248172

Purpose

pSC101-ts plasmid with salicylic acid inducible Lambda-RED operon and constituitive recA expression

Depositor

Thomas Gorochowski

Article

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

Insert

LambdaRED

Expression

Bacterial

Mutation

None

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pSHARK2

Plasmid

#248168

Purpose

pBBR1 plasmid with constituitive lambda-CI (J23101-Bujard-Lambda-CI) and PirWT (PTet-B0033-PirWT) expression

Depositor

Thomas Gorochowski

Article

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

Insert

LambdaCI-PirWT

Expression

Bacterial

Mutation

None

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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Cre Stoplight 2.4

Plasmid

#37402

Purpose

Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

Depositor

Thomas Hughes

Article

Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

Insert

Cre Stoplight 2.4

Tags

ZsGreen1 and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pTKLP-tetA

Plasmid

#71325

Purpose

tetA landing pad template plasmid

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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SM470_EF1A-dEnAsCas12a-T2A-EGFP

Plasmid

#244222

Purpose

Mammalian expression of dEnAsCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LV-TRE-Empty-T2A-dsRedExpress2

Plasmid

#60623

Purpose

Expresses dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Empty

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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