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Showing: 121 - 140 of 3071 results

  1. pML104-LEU2-3

    Plasmid
    #232894
    Purpose
    Plasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    LEU2 gRNA (RER2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232894-plasmid-map-sequence-id-462276

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  2. pMXs-IP- SCAT3.2

    Plasmid
    #172497
    Purpose
    SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    Amcyan
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    MLV LTR
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172497-plasmid-map-sequence-id-340999

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  5. pEGFP-(RTRG)3

    Plasmid
    #69847
    Purpose
    The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.
    Insert
    3 RTRG repeats
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    69847-plasmid-map-sequence-id-125253

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  13. pCDF-casBCDE(-3)

    Plasmid
    #89729
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.
    Depositor
    Konstantin Severinov, Konstantin Kuznedelov
    Article
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a shorter vers…
    Promoter
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89729-plasmid-map-sequence-id-177590

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  20. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Expression
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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Showing: 121 - 140 of 3071 results