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Showing: 121 - 140 of 141943 results

  1. kanMX6-ins1

    Plasmid
    #195038
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    KanR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195038-plasmid-map-sequence-id-384240

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  4. ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA

    Plasmid
    #173213
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173213-plasmid-map-sequence-id-339522

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  11. 36INS2-pBAD_GB

    Plasmid
    #78677
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    78677-plasmid-map-sequence-id-147244

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  12. 36INS2-pBAD_FB

    Plasmid
    #78676
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78676-plasmid-map-sequence-id-147245

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  13. 36INS2-pBAD_EB

    Plasmid
    #78675
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78675-plasmid-map-sequence-id-147246

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  14. 36INS2-pBAD_AB

    Plasmid
    #78674
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78674-plasmid-map-sequence-id-147247

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  18. 36INS1-pBAD_AB

    Plasmid
    #78670
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78670-plasmid-map-sequence-id-147251

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  19. pJBL-RNAINS4

    Plasmid
    #69456
    Purpose
    RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Julius Lucks
    Article
    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
    Insert
    RNA-IN S4 + SFGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    69456-plasmid-map-sequence-id-122578

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  20. pJBL-RNAINS3

    Plasmid
    #69455
    Purpose
    RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Julius Lucks
    Article
    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
    Insert
    RNA-IN S3
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    mutations for S3 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    69455-plasmid-map-sequence-id-122579

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Showing: 121 - 140 of 141943 results