We narrowed to 148,362 results for: ins;

1. (S) insD_ku70 (Q) (SBE139)

   Plasmid

   #187627

   Purpose

   the insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/Q

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (S) insD KU70 (Q)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. (P) insUP_ku70 (SBE138) (B)

   Plasmid

   #187626

   Purpose

   the insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/B

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (P) insUP KU70 (B)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINSPECT:NEAT1-total_SigP:NLuc

   Plasmid

   #197267

   Purpose

   Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Insert

   NEAT1-total homology arms
   

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Expression

   Mammalian

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pegRNA_HEK4_1bp-insertion

   Plasmid

   #201977

   Purpose

   Express a pegRNA used for 1bp insertion at HEK4 site

   Depositor

   Scot Wolfe

   Article

   Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

   Insert

   pegRNA_HEK4_1bp-insertion
   

   Expression

   Mammalian

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC-1-Hairpin_Insulator-4

   Plasmid

   #197693

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   Double hairpin transcriptional insulator with all-frame stops
   

   Expression

   Plant

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. kanMX6-ins1

   Plasmid

   #195038

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Chinchilla–insM

   Plasmid

   #191226

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

   Inserts

   ATP1A1
   ATP1B1
   

   Expression

   Insect

   Mutation

   removed methionine at 122 in the protein sequence

   Promoter

   PH and p10

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sandgrouse–insD

   Plasmid

   #191228

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

   Inserts

   ATP1A1
   ATP1B1
   

   Expression

   Insect

   Mutation

   removed aspartic acid at 121 in the protein seque…

   Promoter

   PH and p10

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA

   Plasmid

   #173213

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_EGFR_p.773_774insH

    Plasmid

    #82855

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Mutation

    Additional H residue inserted between AA 773 and …

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 36INS-pTet_GB

    Plasmid

    #78681

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [G:36INS-pTet:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pTet Promoter
    

    Use

    Synthetic Biology

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSOD1E133insTT-AcGFP1

    Plasmid

    #83441

    Purpose

    Expresses SOD1E133insTT in mammalian cells.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    E133insTT

    Promoter

    CMV

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. 36INS-pTet_FB

    Plasmid

    #78680

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [F:36INS-pTet:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pTet Promoter
    

    Use

    Synthetic Biology

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. 36INS-pTet_EB

    Plasmid

    #78679

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [E:36INS-pTet:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pTet Promoter
    

    Use

    Synthetic Biology

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 36INS-pTet_AB

    Plasmid

    #78678

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [A:36INS-pTet:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pTet Promoter
    

    Use

    Synthetic Biology

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 36INS2-pBAD_GB

    Plasmid

    #78677

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pBAD Promoter
    

    Use

    Synthetic Biology

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 36INS2-pBAD_FB

    Plasmid

    #78676

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pBAD Promoter
    

    Use

    Synthetic Biology

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 36INS2-pBAD_EB

    Plasmid

    #78675

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pBAD Promoter
    

    Use

    Synthetic Biology

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 36INS2-pBAD_AB

    Plasmid

    #78674

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pBAD Promoter
    

    Use

    Synthetic Biology

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 36INS1-pBAD_GB

    Plasmid

    #78673

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [G:36INS1-pBAD:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pBAD Promoter
    

    Use

    Synthetic Biology

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge