We narrowed to 496 results for: transposase

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pSL0341 (pTarget)

Plasmid

#200906

Purpose

Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

pTarget

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2783 (pTnsC-VP64_Vch)

Plasmid

#200896

Purpose

Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsC-VP64

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2669 (pTnsABf_Vch)

Plasmid

#200897

Purpose

Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsA-TnsB

Tags

Internal NLS between TnsA-TnsB fusion

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLV.5’eGFP/sgLMNA

Plasmid

#170546

Purpose

Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

sgRNA targeting 5'- end of LMNA

Use

CRISPR and Lentiviral

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pA-His6-Tn5

Plasmid

#171871

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Protein A and hyperactive Tn5 transposase

Tags

His-tag

Expression

Bacterial

Mutation

WT

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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SB100X in pCAG globin pA

Plasmid

#127909

Purpose

Sleeping Beauty Transposase expression vector driven by the CAG promoter

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

Sleeping Beauty Transposase

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PuroR-T2A-eGFP

Plasmid

#234734

Purpose

Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PuroR-T2A-eGFP

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSL3617 (pCRISPR(BbsI)_Pse)

Plasmid

#200902

Purpose

Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseCRISPR (entry)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLV/eGFP-U6-sgRNA

Plasmid

#170544

Purpose

A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pPBT/EFS-Cas9-PuroTk

Plasmid

#170542

Purpose

Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

PuroTK

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSL3936 (pDonor(AAVS1)_Pse)

Plasmid

#200903

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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TaRGET-ABE-C3.1 ver4.1

Plasmid

#188251

Purpose

Hypercompact adenine base editors

Depositor

Yong-Sam Kim

Article

Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

Insert

TaRGET-ABE3.1

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBac{3XP3::DsRed, Pactin::Ptrsps}

Plasmid

#86862

Purpose

Expresses DsRed in eyes and ubiquitous expression of P-transposase

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Insert

pACNNTNPII-S129A

Expression

Insect

Mutation

S129A

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBac{3XP3::EGFP, Pactin::Ptrsps}

Plasmid

#86861

Purpose

Expresses EGFP in eyes and ubiquitous expression of P-transposase

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Insert

pACNNTNPII-S129A

Expression

Insect

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSL0812 (pCRISPR(BbsI)_Vch)

Plasmid

#200898

Purpose

Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCRISPR (entry)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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TaRGET-ABE-C3.1 ver4.0

Plasmid

#188250

Purpose

Hypercompact adenine base editors

Depositor

Yong-Sam Kim

Article

Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

Insert

TaRGET-ABE3.1

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pT2/CMV-eGFP.PB/PTK

Plasmid

#170540

Purpose

Expresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restored

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

Delta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV.5’eGFP/sgVIM

Plasmid

#170548

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

sgRNA targeting 5'- end of VIM

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_103

Plasmid

#225785

Purpose

This is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.

Depositor

Nili Ostrov

Insert

Ptn5_[Tn5]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_85

Plasmid

#225800

Purpose

This is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.

Depositor

Nili Ostrov

Insert

PmarC9_[Himar]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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