We narrowed to 11,913 results for: pcDNA

1. pCDNA3-V5-PTPN14-PPxY

   Plasmid

   #61006

   Purpose

   Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

   Depositor

   Jianmin Zhang

   Article

   Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

   Insert

   PTPN14 PPxY mutation (PTPN14 Human)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   PPxY mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-optoB2-SS-YFP

   Plasmid

   #75270

   Purpose

   Arrestin independent version of optoB2 with serine mutations at S362A,S363G

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   Arrestin independent version of optoB2
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   serine mutations at S362A,S363G

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-Rs7327-RBD-8h

   Plasmid

   #161823

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-sapphireAKAR-NLS

   Plasmid

   #118467

   Purpose

   cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireAKAR-NLS
   

   Use

   Tags

   Nuclear localization signal (NLS)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4-Xpress-GBF1

   Plasmid

   #206417

   Purpose

   Mammalian expression of hGBF1

   Depositor

   Kazuhisa Nakayama

   Article

   Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

   Insert

   GBF1 (GBF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-sACE2v2.4-IgG1

   Plasmid

   #149668

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.4

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-FRT-TO-FH-Nsp13

   Plasmid

   #157713

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp13 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pcDNA5/FRT/TO-FNTB-HA

   Plasmid

   #86869

   Purpose

   To express FNTB with a HA tag at C terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   FNTB (FNTB Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA4.TO-ORF68-2xCSTREP

   Plasmid

   #136229

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF68

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF68
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Kohinoor 2.0/pcDNA3

    Plasmid

    #182315

    Purpose

    Positively reversibly photoswitchable fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Wazawa et al Microscopy (Oxf). 2021 Aug 9;70(4):340-3

    Insert

    Kohinoor 2.0
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5/mCherry-pBirA-HA

    Plasmid

    #154458

    Purpose

    expresses mCherry-pBirA-HA/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    mCherry-pBirA-HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F, T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-C-terminal CyPet

    Plasmid

    #128026

    Purpose

    Mammalian expression vector with C terminal CyPet tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    CyPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1his p66shc S36A

    Plasmid

    #10973

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    S36A

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-RaACR_687-EYFP

    Plasmid

    #111077

    Purpose

    Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RaACR_687
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-sACE2v2-sfGFP

    Plasmid

    #145173

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3-C1-mCherry-LOVPLD

    Plasmid

    #219559

    Purpose

    ER-targeted LOVPLD

    Depositor

    Jeremy Baskin

    Article

    Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

    Insert

    C1-mCherry-LOVPLD
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3/mArf3(T31N)-HA

    Plasmid

    #79416

    Purpose

    Expresses C-terminally HA-tagged Arf3(T31N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf3 (Arf3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 31 to Asparagine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3/mArf3(Q71L)-HA

    Plasmid

    #79415

    Purpose

    Expresses C-terminally HA-tagged Arf3(Q71L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf3 (Arf3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 71 to Leucine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-dCas9-CibN

    Plasmid

    #60552

    Purpose

    Expresses dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-Flag-LmoCas6

    Plasmid

    #126485

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1/HisC 14-3-3 epsilon wt

    Plasmid

    #11942

    Depositor

    Michael Yaffe

    Article

    Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

    Insert

    Ywhae (Ywhae Rat)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3 ALK4 K234R

    Plasmid

    #80881

    Purpose

    mammalian expression of ALK4 K234R

    Depositor

    Peter ten Dijke, Aristidis Moustakas

    Article

    ten Dijke et al Science. 1994 Apr 1;264(5155):101-4.

    Insert

    ALK4 (ACVR1B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K234R

    Promoter

    CMV

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1 FLIPglu-3.2mDelta13V

    Plasmid

    #18011

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu F16A S112A Delta13
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    F16A, S112A in binding protein

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA Myc-mMEKK2

    Plasmid

    #44158

    Depositor

    Xin Lin

    Article

    Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

    Insert

    Mekk2 (Map3k2 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

25. GLI1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176968

    Purpose

    Mammalian expression vector encoding GLI1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA4.TO-ORF61-2xCSTREP

    Plasmid

    #136221

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF61

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF61
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HA-GFP1-10-actin-pcDNA4-TO

    Plasmid

    #118370

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Tags

    HA-GFP1-10

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA3 Flag Jnk1a1MKK7B2

    Plasmid

    #19731

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    Jnk1a1MKK7b2 (Map2k7, MAPK8 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3 HA Sam68 G178E

    Plasmid

    #17689

    Depositor

    David Shalloway

    Article

    Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

    Insert

    Sam68 (Khdrbs1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G178E

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pcDNA5-FRT-TO-Nsp12-HF

    Plasmid

    #157711

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp12-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pcDNA3 Flag PA1

    Plasmid

    #15559

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    PA1 (PAGR1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.1(-) Hu 4-1BB(CD137)-Fc

    Plasmid

    #183251

    Purpose

    Soluble expression of human 4-1BB (CD137)-Fc. Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    41BB Fc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-TOP luciferase

    Plasmid

    #26611

    Depositor

    Jie Chen

    Article

    Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

    Insert

    eEF2 5'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pcDNA5-FRT-TO-Nsp8-HF

    Plasmid

    #157696

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pcDNA3.1_mGFP-FGFR3-K650E

    Plasmid

    #191757

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1948A>G (p.K650E) + silent mutation c.1938C&…

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-TORCAR-NES

    Plasmid

    #175015

    Purpose

    Cytosol-targeted TORCAR.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    TORCAR-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA3 IRF9-STAT1C

    Plasmid

    #37543

    Depositor

    Curt Horvath

    Article

    Kraus et al J Biol Chem. 2003 Apr 11;278(15):13033-8

    Insert

    IRF-9 STAT1 C (IRF9, STAT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

    Plasmid

    #169326

    Purpose

    Expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-CARMA2sh-FLAG

    Plasmid

    #97071

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1 PA-mCit-BAD

    Plasmid

    #113459

    Purpose

    ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    BCL2 associated agonist of cell death (BAD Human)
    

    Use

    Tags

    ProteinA-mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge