We narrowed to 2,115 results for: D10

1. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

   Plasmid

   #102889

   Purpose

   PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR1A mKate2-hu_ncOGT WT

   Plasmid

   #154282

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Expression

   Mutation

   WT OGT

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV_Npu-BE3.9max N-terminal

   Plasmid

   #137171

   Purpose

   Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-BE3.9max_N-terminal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV_Npu-ABEmax N-terminal

   Plasmid

   #137173

   Purpose

   Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-ABEmax_N-terminal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

   Plasmid

   #107319

   Purpose

   Expresses SpCas9 fused to dSaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xFLAG-dCas9/pMXs-IG

   Plasmid

   #51258

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_eA3A-nCas9

   Plasmid

   #163535

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-hA3F-BE3

   Plasmid

   #113414

   Purpose

   Expresses hA3F-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3F-BE3 (APOBEC3F Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC_C_LAMP1

   Plasmid

   #111669

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid

    #98041

    Purpose

    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    Sa-dCas9-NLS-3xFLAG
    

    Use

    CRISPR

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    D10A + H557A

    Promoter

    CMV

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT82_GalL_nCDA1Δ198-BE3

    Plasmid

    #145046

    Purpose

    Expresses nCDA1Δ198-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ198-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-198AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUF1-dCas9-Sir2a

    Plasmid

    #122510

    Purpose

    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Insert

    dCas9-Sir2a
    

    Use

    CRISPR

    Tags

    3*FLAG

    Expression

    Mutation

    dCas9(D10A,H840A)

    Promoter

    PfHsp86

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CABE-T1.17

    Plasmid

    #193260

    Purpose

    Expression of CABE-T1.17 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T1.17
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ266

    Plasmid

    #164713

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Semaphorin7A-CD4d3+4-bio

    Plasmid

    #73115

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Semaphorin7A with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Semaphorin 7A (SEMA7A Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_Anc689-nCas9

    Plasmid

    #163526

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT25_GalL_BE3

    Plasmid

    #143736

    Purpose

    Expresses BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR1A hu_ncOGT WT (open N-term)

    Plasmid

    #155197

    Purpose

    Entry vector encoding non-tagged O-GlcNAc Transferase with open N-terminus

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    WT OGT with no start codon, in-frame with gateway…

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. HEpl017

    Plasmid

    #179992

    Purpose

    pCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    TadA8e-dSiCas12i(D1049A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti PGK OGT (Neo)

    Plasmid

    #154289

    Purpose

    Lentiviral vector encoding full length wildtype human OGT

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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