We narrowed to 2,962 results for: ER;

1. pET SUMO-syt7 (136-403)

   Plasmid

   #213621

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 (136-403)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 (136-403) (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET SUMO-syt1 5W

   Plasmid

   #213623

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 5W

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 5W (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   M173W, F231W, F234W, C277A, V304W, Y364W, I367C

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET SUMO-syt1 s7+

   Plasmid

   #213624

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 s7+

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 s7+ (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET SUMO-syt7 C2AB - 4A

   Plasmid

   #213625

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 C2AB - 4A

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 C2AB - 4A (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   F167A, F229A, I298A, L361A

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET SUMO-syt7 C2AB-2A

   Plasmid

   #213627

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 C2AB-2A (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   I298, L361A

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFSynW pHluorin-SYT9 IRES mRuby3

   Plasmid

   #195698

   Purpose

   Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFSynW Syt9 IRES mRuby3

   Plasmid

   #195700

   Purpose

   Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   encodes only the first 68 amino acids of Tac1

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFSynW SYT9 D197, 199N IRES GFP

   Plasmid

   #195701

   Purpose

   Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   full-length mouse SYT9 with D197, 199N mutations

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFSynW SYT9 D330, 332N IRES GFP

   Plasmid

   #195702

   Purpose

   Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   full-length mouse SYT9 with D330, 332N mutations

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX SYT9 C2AB D197,199,330,332N

    Plasmid

    #195706

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 104-386 of mouse SYT9 with D1…

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EGFP-ST-P2A-pSC-CAAX

    Plasmid

    #207637

    Purpose

    A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSC-cUnaG-LPETG

    Plasmid

    #207635

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-Best1-Txnip(C247S_LL351-352AA)

    Plasmid

    #206344

    Purpose

    AAV plasmid expressing mutant TXNIP in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    C247S, L351A, L352A

    Promoter

    human Best1

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-dora[B]_rescue_construct

    Plasmid

    #190638

    Purpose

    Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Changed threonine 295 to methionine

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-dora[A]_rescue_construct

    Plasmid

    #190640

    Purpose

    Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFRTpsiCHECK_SEAP

    Plasmid

    #154461

    Purpose

    Expression of SEAP and firefly luciferase

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CTAG_eGFP-GHtag-HDEL

    Plasmid

    #136096

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJS697

    Plasmid

    #168778

    Purpose

    Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Type

    Empty backbone

    Use

    Tags

    Cmyc and HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ad-HisFlag-USP19ER

    Plasmid

    #155244

    Purpose

    for generating adenoviruses expressing the ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Adenoviral

    Tags

    6XHis-3XFlag

    Expression

    Mutation

    H118R- please see dep. comments

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-HisFlag-rUSP19ER-CAmut

    Plasmid

    #155237

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge