We narrowed to 2,903 results for: human cdnas

1. pcDNA3-Flag-NSF/T645A

   Plasmid

   #74936

   Purpose

   Mammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)

   Depositor

   Elisa Greggio

   Article

   Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

   Insert

   NSF (NSF Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T645A (mutation in the D2 domain of the protein)

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

   Plasmid

   #205603

   Purpose

   Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

   Depositor

   Tarun Kapoor

   Article

   Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

   Insert

   γ-tubulinWT-RNAi-resistant-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G2228A, C2231T, C2237T, G2240A

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 CIM5 (Rpt-1) GFP

   Plasmid

   #16074

   Depositor

   Erin Schuman

   Article

   Bingol et al Nature. 2006 Jun 29. 441(7097):1144-8.

   Insert

   CIM5 (RPT1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-hPC1_MTS_V4146I-eGFP

   Plasmid

   #108405

   Purpose

   Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Mouse)
   

   Use

   Tags

   3xHA and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-hPC1_MTS_L4137P-eGFP

   Plasmid

   #108403

   Purpose

   Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Mouse)
   

   Use

   Tags

   3xHA and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-MUC2 D1 with cleavable His tag

   Plasmid

   #181813

   Purpose

   Expresses human MUC2 D1 with cleavable His tag, residues 21-389

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Muc2 D1 (MUC2 Human)
   

   Use

   Tags

   6xHistadine followed by TEV cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-HsPcdh21-Myc-Flag-His

   Plasmid

   #210818

   Purpose

   Mammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Protocadherin 21 (CDHR1 Human)
   

   Use

   Tags

   Myc, 3x Flag, 10x His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5 FRT TO Myc ArA K162R

   Plasmid

   #59805

   Purpose

   Allows the integration of Myc ArA K162R in the genome and Tet-inducible expression

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora A (AURKA Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   K162R, kinase mutant

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 Flag MFF iso2 S172A

   Plasmid

   #74392

   Purpose

   Flag tagged human S172A MFF isoform 2 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 172 Ala (numbering based on MFF isoform 1)

   Promoter

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5 FRT TO Myc ArB WT RNAiR

    Plasmid

    #59806

    Purpose

    Allows the integration of Myc ArB in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora B (AURKB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    RNAi resistant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5 FRT TO Myc ArC Long WT

    Plasmid

    #59809

    Purpose

    Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora C (AURKC Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 Flag MFF iso1 S172A

    Plasmid

    #74391

    Purpose

    Flag tagged human S172A MFF isoform 1 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta exon 1 (1-26) and Ser 172 Ala.

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5 TO FRT Myc ArB K106R

    Plasmid

    #59807

    Purpose

    Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora B (AURKB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K106R, kinase mutant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5 FRT TO Myc ArC K72R

    Plasmid

    #59811

    Purpose

    Allows the integration of Myc ArC K72R in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora C (AURKC Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K72R, kinase mutant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 Flag MFF iso4 S172A

    Plasmid

    #74393

    Purpose

    Flag tagged human S172A MFF isoform 4 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA 3.1 myc-Rab10-mito

    Plasmid

    #208367

    Purpose

    Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    myc-Rab10-mito (RAB10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wildtype

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 FRT TO myc Separase S1226A/ C2029A

    Plasmid

    #59823

    Purpose

    Allows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S1226A/ C2029A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

    Plasmid

    #205604

    Purpose

    Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

    Depositor

    Tarun Kapoor

    Article

    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

    Insert

    γ-tubulinΔGTP-RNAi-resistant-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G2228A, C2231T, C2237T, G2240A, N229A

    Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid

    #61365

    Purpose

    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    Nm-dCas9-p300 Core (EP300 Neisseria meningiditis, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D16A, D587A, H588A, N611A in Nm Cas9

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

    Plasmid

    #115788

    Purpose

    Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    LRP5 (LRP5 Human)
    

    Use

    Tags

    3xflag

    Expression

    Mammalian

    Mutation

    contains silent mutation that creates an internal…

    Promoter

    CMV

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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