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  4. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid
    #37184
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Use
    Tags
    FokI nuclease
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    37184-plasmid-map-sequence-id-46727

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  5. pREPK219H

    Plasmid
    #51492
    Purpose
    pREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Insert
    Rep/RepA
    Use
    Plant expression t-dna
    Tags
    Expression
    Mutation
    changed lysine 219 to histidine
    Promoter
    2x35S
    Available since
    May 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    51492-plasmid-map-sequence-id-78586

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  13. pNuc-TrxRFP1

    Plasmid
    #98998
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization
    Depositor
    Huiwang Ai
    Article
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Insert
    TrxRFP1 and nuclear localization sequence
    Use
    Tags
    His-tag and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    98998-plasmid-map-sequence-id-190366

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  19. pLSLGUS

    Plasmid
    #51517
    Purpose
    pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GUS
    3kb filler sequence
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51517-plasmid-map-sequence-id-78696

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Showing: 1381 - 1400 of 3518 results