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20XUAS IVS Jaws mVenus_tr

Plasmid

#111552

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mVenus_tr

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-NLS-VHL-miniTurbo-3xFLAG

Plasmid

#248670

Purpose

To generate inducible Flp-In cells expressing Cterm tagged miniTurbo full length VHL for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL (VHL Human)

Tags

FLAG-miniTurbo and NLS

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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cBEST2

Plasmid

#234658

Purpose

Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

Depositor

Mingzi M. Zhang

Article

Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

Inserts

spCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

kasO*17tss and kasOp*

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-AHR-shRNA1

Plasmid

#166889

Purpose

Lentivirus for inducible knockdown of human AHR

Depositor

Roland Friedel

Article

Halawani et al bioRxiv 2023.11.04.565649. doi: https://

Insert

AHR shRNA (AHR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF_AaRaf1/Raf2/RbcX2/BSD2

Plasmid

#229507

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX2,BSD2

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
Rubisco accumulation factor 2
RbcX2
BSD2

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF_AaRaf1/Raf2/RbcX1/BSD2

Plasmid

#229508

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX1,BSD2

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
Rubisco accumulation factor 2
RbcX1
BSD2

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF_AaRaf2/RbcX2/BSD2/RbcX1

Plasmid

#229512

Purpose

Expresses Anthoceros agrestis chaperones: Raf2,RbcX2,BSD2,RbcX1

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 2
RbcX2
BSD2
RbcX1

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF_AaRaf1/Raf2/RbcX2/RbcX1

Plasmid

#229514

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX2,RbcX1

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
Rubisco accumulation factor 2
RbcX2
RbcX1

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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T40A+Q54A

Plasmid

#217913

Purpose

A plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutations

Depositor

Cole DeForest

Article

Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

Insert

PXP T40A+Q54A

Tags

6x His (N and C term)

Expression

Bacterial

Mutation

Threonine at position 41 and 253 mutated to Alani…

Promoter

T5

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Mutation

Cas4 domain (K81A)

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut2x

Plasmid

#182254

Purpose

Human PDAP1 3` UTR region mutated in 2x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 2 binding sites for mir-150

Use

Luciferase

Expression

Mammalian

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1x

Plasmid

#182253

Purpose

Human PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 1 binding site for mir-150

Use

Luciferase

Expression

Mammalian

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3x

Plasmid

#182255

Purpose

Human PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 3 binding sites for mir-150

Use

Luciferase

Expression

Mammalian

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-anti-MIR328-3p

Plasmid

#153319

Purpose

Expresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen with MIR328-3p sponge

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-anti-MIR211-5p

Plasmid

#153318

Purpose

Expresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen with MIR211-5p sponge

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGA_pET28

Plasmid

#167326

Purpose

Expresses TFAM HMGA mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA domain (43-122)

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGA_linker_pET28

Plasmid

#167327

Purpose

Expresses TFAM HMGA+linker mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA and linker doma…

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGB_ctail_pET28

Plasmid

#167330

Purpose

Expresses TFAM HMGB+C-tatil mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGB and C-tail doma…

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_dC_pET28

Plasmid

#167328

Purpose

Expresses TFAM dC-tail mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA, linker and HMG…

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgALCAM

Plasmid

#83929

Purpose

Lentiviral vector expressing an sgRNA targeting ALCAM. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgALCAM

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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