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We narrowed to 6,758 results for: sas

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Showing: 1381 - 1400 of 6758 results

  3. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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  5. FUG-T2A-Cas9

    Plasmid
    #75346
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75346-plasmid-map-sequence-id-142581

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  13. EC71167

    Plasmid
    #154066
    Purpose
    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    Use
    Synthetic Biology
    Mutation
    All BsaI, Esp3I and BPiI restriction sites were r…
    Promoter
    Zea mays Ubiquitin Promoter
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154066-plasmid-map-sequence-id-312954

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Showing: 1381 - 1400 of 6758 results