We narrowed to 10,632 results for: t7

1. pGEMHE-MmCENP-O-GFP

   Plasmid

   #205178

   Purpose

   Expresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

   Insert

   CENP-O (Cenpo Mouse)
   

   Use

   In vitro transcription

   Tags

   5 glycines linker followed by GFP

   Expression

   Mammalian

   Mutation

   Wild type mouse CENP-O

   Promoter

   T7

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET PI-SceI C-His

   Plasmid

   #195610

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag

   Depositor

   Frederick Gimble

   Article

   He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

   Insert

   PI-SceI
   

   Expression

   Bacterial

   Mutation

   T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…

   Promoter

   T7

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGG438

   Plasmid

   #165486

   Purpose

   Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   D1135V, S1136R, D1332K, and R1333G mutations in S…

   Promoter

   CMV and T7

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBB0238-TEV-His12

   Plasmid

   #137038

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66635.2
   

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1-neoR

   Plasmid

   #117048

   Purpose

   In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   neoR
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETa-Amrose v2

   Plasmid

   #79538

   Purpose

   pRSETa (Invitrogen) plasmid expressing Amrose variant 2

   Depositor

   Randolph Caldwell

   Article

   Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

   Insert

   Amrose v2
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. SRAcode_in_pUC57_v3(419-999)

   Plasmid

   #49824

   Purpose

   for transcribing SRA nucl 419-999 of NM_001035235 with T7

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator RNA coding (SRA1 Human)
   

   Expression

   Bacterial

   Mutation

   KpnI site at 5' start of RNA coding seq

   Promoter

   T7

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. SRAcode_in_pUC57_v3(767-999)

   Plasmid

   #49825

   Purpose

   for transcribing SRA nucl 767-999 of NM_001035235 with T7

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator RNA coding (SRA1 Human)
   

   Expression

   Bacterial

   Mutation

   KpnI site at 5' start of RNA coding seq

   Promoter

   T7

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. SRAcode_in_pUC57_v3(419-771)

   Plasmid

   #49823

   Purpose

   for transcribing SRA nucl 419-771 of NM_001035235 with T7

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator RNA coding (SRA1 Human)
   

   Expression

   Bacterial

   Mutation

   KpnI site at 5' start of RNA coding seq

   Promoter

   T7

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-LIC

    Plasmid

    #62303

    Purpose

    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAIP4_1UXZ

    Plasmid

    #234166

    Purpose

    Heterologous protein expression of cellulase B in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1UXZ
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. His-GST

    Plasmid

    #215169

    Purpose

    Encodes the synthetic glutathionine S-transferase with N-terminal His tag

    Depositor

    Bhuvana Shanbhag, Travis Beddoe

    Insert

    His-GST
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7 lac

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHN767

    Plasmid

    #178884

    Purpose

    Expression of bacterial PNGase

    Depositor

    Kazuhiro Abe

    Insert

    Peptide:N-glycosidase F
    

    Tags

    GST, His8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOLENTE

    Plasmid

    #80683

    Purpose

    DEST vector for cell-free in vitro expression

    Depositor

    Nico Dissmeyer

    Type

    Empty backbone

    Use

    Synthetic Biology; In vitro transcription and tra…

    Expression

    Mammalian

    Promoter

    T7 and SP6

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3' EGFP pXOON

    Plasmid

    #48699

    Purpose

    Creates a 3' Fusion of EGFP to insert for expression in Xenopus oocytes or mammalian cells

    Depositor

    Dmitri Boudko

    Type

    Empty backbone

    Use

    Xenopus oocyte expression

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZIKV_KX377337.1_NS2B

    Plasmid

    #234404

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    NS2B_ZIKV
    

    Tags

    MGSSHHHHHHSSGLVPRGS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Denv2_GU289914.1_NS2B

    Plasmid

    #234401

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    NS2B_DENV2
    

    Tags

    MGSSHHHHHHSSGLVPRGS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. CHICKV_KY575571.1_NSP2_Pro_NHis_CAvi

    Plasmid

    #234394

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp2_CHICKV
    

    Tags

    MHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHE

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. CHICKV_KY575571.1_NSP2_Hel_NAviCHis

    Plasmid

    #234397

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp2_CHICKV
    

    Tags

    MHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHE

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Denv4_KJ160504.1_NS2B

    Plasmid

    #234402

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    NS2B_DENV4
    

    Tags

    MGSSHHHHHHSSGLVPRGS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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