We narrowed to 1,440 results for: tetracycline

1. B4GALT1-Dox

   Plasmid

   #124633

   Purpose

   Doxycycline-inducible B4GALT1 expression in mammalian cells

   Depositor

   Ron Weiss

   Article

   Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

   Inserts

   Beta-(1,4)-Galactosyltransferase 1
   Tetracycline repressor A3 mutant
   TagFP635
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight and hEF1a

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST

   Plasmid

   #182547

   Purpose

   Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificity

   Depositor

   Robert de Vries

   Article

   Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

   Insert

   H5_Vietnam_1203_04_Y161A
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   Y161A mutant that confers exclusive NeuAc to NeuG…

   Promoter

   CMV

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

   Plasmid

   #36881

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tTA2
   beta-globin intron
   Neo
   tdT-3Myc
   insulator
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

   Plasmid

   #157701

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3.1/TO-myc-BirA*-cCE

   Plasmid

   #112713

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   Rngtt (Rngtt Mouse)
   

   Use

   Tags

   BirA* (BirA R118G), HIV Rev nuclear localization …

   Expression

   Mammalian

   Mutation

   Silent mutation at Arg 132 (CGT to CGC), deleted …

   Promoter

   CMV

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-TRE-scFVDnmt3A_bGHpA

   Plasmid

   #177355

   Purpose

   AAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   598–908 aa of mouse Dnmt3a

   Promoter

   Tetracycline-dependent promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-TRE-scFVtet1_bGHpA

   Plasmid

   #177357

   Purpose

   AAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   N terminal domain of human Tet1

   Promoter

   Tetracycline-dependent promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-TRE-scFVtet2_bGHpA

   Plasmid

   #177358

   Purpose

   AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 2 (TET2 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   Catalytic domains of human TET2 (1129–1936 and 14…

   Promoter

   Tetracycline-dependent promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-syn-FLEX-tTA

   Plasmid

   #178516

   Purpose

   AAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expression

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4/TO-bio-myc-cCE

    Plasmid

    #112711

    Purpose

    For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Use

    Tags

    HIV Rev nuclear localization signal (NES), Stop c…

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV with Tet repressor binding sites

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMH005_phiC31_neo-core-5xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid

    #179433

    Purpose

    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    coreHS4-TRE-cit-coreHS4-mch (DC)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL008_phiC31_neo-5xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179426

    Purpose

    Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-5kb-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMH010_phiC31_neo-5xTetO-pEF-H2B-mCitrine-1.2kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179427

    Purpose

    Dual fluorescent reporter construct with 1.2kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-1.2kb-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-idCas9-KRAB-Hygro donor

    Plasmid

    #199621

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Insert

    AAVS1-idCas9-KRAB-Hygro
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS3 (CK2alpha, CK2beta)

    Plasmid

    #27092

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha (CSNK2A1 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional tet-responsive promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPos-Ma-CmR-TAG111

    Plasmid

    #197571

    Purpose

    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    111TAG and 8TAG/112TAG

    Promoter

    AraC, T7, cat promoter (constitutive), and lpp (c…

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ134-STU-As

    Plasmid

    #138103

    Purpose

    Golden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid

    #176836

    Purpose

    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    See Depositor Comments Below

    Promoter

    Tet operator (tetracycline inducible expression)

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ131-STU-Lb

    Plasmid

    #138096

    Purpose

    Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ134-STU-Lb

    Plasmid

    #138105

    Purpose

    Golden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge