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pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Article

Kim et al bioRxiv. 2023 Jan 20:2023.01.19.524797.

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TCF7L2_pLX307

Plasmid

#98373

Purpose

Lentiviral expression of TCF7L2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TCF7L2 (TCF7L2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Isoform X25

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pADH147

Plasmid

#90991

Purpose

NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

gRNA conserved, FRT, DS_HIS1

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-Hygro-GFP

Plasmid

#61215

Purpose

Retrovirus expressing GFP

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

GFP

Use

Retroviral

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-ngRNA+41_EF1a-puroR

Plasmid

#207358

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 +41 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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ARR3tk-eGFP/SV40-mCherry

Plasmid

#132360

Purpose

To measure transcriptional activity of Androgen Receptor

Depositor

Charles Sawyers

Article

Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

Insert

AR responsive elements (AR Human)

Use

Lentiviral

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-COL1A1

Plasmid

#66602

Purpose

Expresses procollagen 1 alpha 1 as a GFP fusion

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

Insert

COL1A1 (COL1A1 Human)

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G

Plasmid

#194457

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-CD19 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-eyfp

Plasmid

#65031

Purpose

GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

eyfp

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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SaCas9 TadCBEd

Plasmid

#193845

Purpose

Express TadCBEd (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd with SaCas9

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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