165,567 results

1. pHTNW

   Plasmid

   #136403

   Purpose

   Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57.1 N-term GFP tTA

   Plasmid

   #107551

   Purpose

   All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

   Depositor

   David Sabatini

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP (optional)

   Expression

   Mammalian

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-UTX-F

   Plasmid

   #17438

   Purpose

   Expression of UTX in mammalian cells

   Depositor

   Kai Ge

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

   Insert

   UTX (KDM6A Human)
   

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM5-WT PHGDH

   Plasmid

   #83901

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. MMLV-CAG-SADB19_oG-IRES-Puro

   Plasmid

   #172367

   Purpose

   Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   oG + Puro
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. NXPE1_pcDNA3.4-TOPO

   Plasmid

   #226841

   Purpose

   Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.

   Depositor

   Bert Vogelstein

   Insert

   NXPE1 (NXPE1 Human)
   

   Tags

   x6 HIS and an IL2 secretion signal both N termina…

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G

   Plasmid

   #194464

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEFBOS_CreIRESpuro

   Plasmid

   #183812

   Purpose

   Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

   Depositor

   Ed Stanley

   Article

   Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

   Insert

   Cre recombinase
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. DsRed2-N1

    Plasmid

    #54493

    Purpose

    Localization: N1 Cloning Vector, Excitation: 558, Emission: 583

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    DsRed2

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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